cDNA Clone Ubiquitin

• Plasmid: pHisTEV30a Ubiquitin M-2C N60A
• Parent Plasmid: pHisTEV30a
• Expressed: 6His TEV Ubiquitin M-2C N60A
• Genbank: N/A
• Tag: 6His
• Species: Human

Sequence of Insert

CATATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGACCGAAAACCTGTATTTTCAGGGCGCCTGCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACGCTATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAAAGCTTGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGACGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYAIQKESTLHLVLRL
RGG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU61213
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to NCBI reference sequence there i a silent mutation at R73 (a220c). Clone contains Cys residue two amino acids upstream of Ubiquitin start - introduced for labelling of ubiquitin with fluorescent dye.
• Low Temp: No