cDNA Clone TRIM16

• Synonyms: EBBP
• Plasmid: pet15D TRIM16
• Parent Plasmid: pET15D
• Expressed: 6His TRIM16
• Genbank: NM_001348119.1
• Tag: 6His

Sequence of Insert

gaattcATGGCTGAGTTGGATCTAATGGCTCCAGGGCCACTGCCCAGGGCCACTGCTCAGCCCCCAGCCCCTCTCAGCCCAGACTCTGGGTCACCCAGCCCAGATTCTGGGTCAGCCAGCCCAGTGGAAGAAGAGGACGTGGGCTCCTCGGAGAAGCTTGGCAGGGAGACGGAGGAACAGGACAGCGACTCTGCAGAGCAGGGGGATCCTGCTGGTGAGGGGAAAGAGGTCCTGTGTGACTTCTGCCTTGATGACACCAGAAGAGTGAAGGCAGTGAAGTCCTGTCTAACCTGCATGGTGAATTACTGTGAAGAGCACTTGCAGCCGCATCAGGTGAACATCAAACTGCAAAGCCACCTGCTGACCGAGCCAGTGAAGGACCACAACTGGCGATACTGCCCTGCCCACCACAGCCCACTGTCTGCCTTCTGCTGCCCTGATCAGCAGTGCATCTGCCAGGACTGTTGCCAGGAGCACAGTGGCCACACCATAGTCTCCCTGGATGCAGCCCGCAGGGACAAGGAGGCTGAACTCCAGTGCACCCAGTTAGACTTGGAGCGGAAACTCAAGTTGAATGAAAATGCCATCTCCAGGCTCCAGGCTAACCAAAAGTCTGTTCTGGTGTCGGTGTCAGAGGTCAAAGCGGTGGCTGAAATGCAGTTTGGGGAACTCCTTGCTGCTGTGAGGAAGGCCCAGGCCAATGTGATGCTCTTCTTAGAGGAGAAGGAGCAAGCTGCGCTGAGCCAGGCCAACGGTATCAAGGCCCACCTGGAGTACAGGAGTGCCGAGATGGAGAAGAGCAAGCAGGAGCTGGAGAGGATGGCGGCCATCAGCAACACTGTCCAGTTCTTGGAGGAGTACTGCAAGTTTAAGAACACTGAAGACATCACCTTCCCTAGTGTTTACGTAGGGCTGAAGGATAAACTCTCGGGCATCCGCAAAGTTATCACGGAATCCACTGTACACTTAATCCAGTTGCTGGAGAACTATAAGAAAAAGCTCCAGGAGTTTTCCAAGGAAGAGGAGTATGACATCAGAACTCAAGTGTCTGCCGTTGTTCAGCGCAAATATTGGACTTCCAAACCTGAGCCCAGCACCAGGGAACAGTTCCTCCAATATGCGTATGACATCACGTTTGACCCGGACACAGCACACAAGTATCTCCGGCTGCAGGAGGAGAACCGCAAGGTCACCAACACCACGCCCTGGGAGCATCCCTACCCGGACCTCCCCAGCAGGTTCCTGCACTGGCGGCAGGTGCTGTCCCAGCAGAGTCTGTACCTGCACAGGTACTATTTTGAGGTGGAGATCTTCGGGGCAGGCACCTATGTTGGCCTGACCTGCAAAGGCATCGACCGGAAAGGGGAGGAGCGCAACAGTTGCATTTCCGGAAACAACTTCTCCTGGAGCCTCCAATGGAACGGGAAGGAGTTCACGGCCTGGTACAGTGACATGGAGACCCCACTCAAAGCTGGCCCTTTCCGGAGGCTCGGGGTCTATATCGACTTCCCGGGAGGGATCCTTTCCTTCTATGGCGTAGAGTATGATACCATGACTCTGGTTCACAAGTTTGCCTGCAAATTTTCAGAACCAGTCTATGCTGCCTTCTGGCTTTCCAAGAAGGAAAACGCCATCCGGATTGTAGATCTGGGAGAGGAACCCGAGAAGCCAGCACCGTCCTTGGTGGGGACTGCTCCCTAGgcggccgc

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSKLEFMAELDLMAPGPLPRATAQPPAPLSPDSGSPSPDSGSASPVEEEDVGSSEKLGRETEEQDSDSAEQGDPAGEGKEVLCDFCLDDTRRVKAVKSCLTCMVNYCEEHLQPHQVNIKLQSHLLTEPVKDHNWRYCPAHHSPLSAFCCPDQQCICQDCCQEHSGHTIVSLDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pet15D TRIM16125.88 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66356
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Direct digest of RTPCR product
• Low Temp: No