cDNA Clone UBE3a

• Synonyms: AS; ANCR; E6-AP; HPVE6A; EPVE6AP
• Plasmid: pGEX-TEV-UBE3A Q495-Leu852 (end).
• Parent Plasmid: pGEX-TEV
• Expressed: GST-tev-ube3a 495-852
• Genbank: NP_001341435.1
• Tag: GST
• Species: Human

Sequence of Insert

ggatccCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATAT
CATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTG
CAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTT
GATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAAT
CTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGT
TTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATT
GGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACA
TTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTC
GTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTA
TTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGAT
ATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAA
ATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAAT
CTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGC
TTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTACT
TATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTA
GATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAG
GGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAG
ATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCA
CCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCC
AGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTGCTTTTAC
TTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCC
ATCACGTATGCCAAAGGATTTGGCATGCTGTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSQLNPYLRLKVRRDHIIDDALV
RLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIG
MFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVY
RKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQTDLF
GNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFH
MVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRDSVLIR
EFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLP
TSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX-TEV-UBE3A Q495-Leu852 (end).480.22 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65569
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 20757; Numbering for isoform 1 NCBI ref
• Low Temp: No