cDNA Clone NUAK2

• Synonyms: SNARK
• Plasmid: pSCB NUAK2 D237A (D193A KD) new FL Sal Not
• Parent Plasmid: pSC-B
• Genbank: NM_030952.3, NP_112214.3
• Species: Human

Sequence of Insert

GTCGACATGCTGTGCTTTACTGCGCGCTCTGGTACTGCTGTGGCTCCCCGTCCTGGTGCGGGACCTGTGCCCCGCGCTTCAGCCCTCCCCGCACAGCCTACTGATTCCCCTGCCGCCCTTGCTCACCTCCTGCTCGCCATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCCCGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGAAGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGCAAAGGCACCTACGGGAAGGTGAAGAAGGCGCGGGAGAGCTCGGGGCGCCTGGTGGCCATCAAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATCTGATGCACATACGGAGGGAGATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGAAGTGTTTGAGAACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTTATGACTACATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCATTTCTTCCGGCAGATCGTCTCTGCCGTGCACTATTGCCATCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAACATCCTCTTGGATGCCAATGGGAATATCAAGATTGCTGCCTTCGGCCTCTCCAACCTCTACCATCAAGGCAAGTTCCTGCAGACATTCTGTGGGAGCCCCCTCTATGCCTCGCCAGAGATTGTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCTGGTCCCTGGGTGTTCTCCTCTACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGACCATAAGATCCTAGTGAAACAGATCAGCAACGGGGCCTACCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATCCGGTGGCTGTTGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCACTGGTGGGTCAACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGTGGGCACCCTGGCAGTGACTCTGCCCGCGCCTCCATGGCTGACTGGCTCCGGCGTTCCTCCCGCCCCCTCCTGGAGAATGGGGCCAAGGTGTGCAGCTTCTTCAAGCAGCATGCACCTGGTGGGGGAAGCACCACCCCTGGCCTGGAGCGCCAGCATTCGCTCAAGAAGTCCCGCAAGGAGAATGACATGGCCCAGTCTCTCCACAGTGACACGGCTGATGACACTGCCCATCGCCCTGGCAAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAAGGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGCAGGCTGCCCCCCTGCTCCCCAAGAAGGGCATTCTCAAGAAGCCCCGACAGCGCGAGTCTGGCTACTACTCCTCTCCCGAGCCCAGTGAATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGTGGGGATCCCAAGGAGCAGAAGCCTCCGCAAGCTTCAGGGCTCCTCCTCCATCGCAAAGGCATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTAGAGCTCGCGGCCCCCACCACCTTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAGCCGACCCTCAGGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTTGACCAGCTGGACTTGCCTGAACGGCTCCCAGAGCCCCCACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTTGAGGAGCCCCCCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTGGGGGACAGCTGCTTTTCCCTGACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCACTGAGGGTCTGCTCAAAGCTCACCTGAGCGGCCGC

Amino Acid Sequence

MLCFTARSGTAVAPRPGAGPVPRASALPAQPTDSPAALAHLLLAMESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFL
ETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHFF
RQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIAAFGLSNLYHQGKFLQTFCGSPLYASPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDHK
ILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFKQ
HAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAEGVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRE
SGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQ
LDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU69137
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D237A kinase dead (equivalent to D193A in earlier truncated isoform). Full-length updated NCBI NM_030952.3 (ie. includes N-terminal extension); silent changes at G239 (GGT > GGC), L538 (CTG > CTC), L557 (TTG > TTA)
• Low Temp: No