cDNA Clone UBE2L3

• Plasmid: pET156-UBE2L3-SUMO2 (TAG11)
• Parent Plasmid: pET156
• Expressed: UBE2L3-SUMO2 (TAG11)-6His
• Genbank: NA
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ccATGGCCGCCTCTCGGAGACTGATGAAGGAACTGGAAGAGATCCGGAAG
TGCGGCATGAAGAACTTCCGGAACATCCAGGTGGACGAGGCCAACCTGCT
GACATGGCAGGGACTGATCGTGCCCGACAATCCTCCTTACGACAAGGGCG
CCTTCAGAATCGAGATCAACTTCCCCGCCGAGTATCCCTTCAAGCCTCCT
AAGATCACCTTCAAGACCAAGATCTATCACCCCAACATCGACGAGAAGGG
CCAAGTGTGCCTGCCTGTGATCAGCGCCGAGAATTGGAAGCCTGCCACCA
AGACCGACCAAGTGATCCAGTCTCTGATCGCCCTGGTCAACGACCCTCAG
CCTGAACATCCTCTGAGAGCCGATCTGGCCGAAGAGTACAGCAAGGACCG
GAAGAAGTTCTGCAAGAACGCCGAGGAATTCACCAAGAAGTACGGCGAGA
AGAGGCCCGTGGATGGTGGATCTGGCGGAGGAAGCGGTGGCGGAAGCGGC
GGAGGTTCAGGCGGAGGATCTGAGAACCTGTACTTCCAAGGCATGGCCGA
TGAGAAGCCCAAAGAAGGCGTGTAGACCGAGAACAACGACCACATCAACC
TGAAGGTGGCCGGACAGGATGGCAGCGTGGTGCAGTTCAAGATCAAGCGG
CACACCCCTCTGAGCAAGCTCATGAAGGCCTACTGCGAGAGACAGGGCCT
GAGCATGCGGCAGATCCGGTTCAGATTTGACGGCCAGCCTATCAACGAGA
CAGACACCCCTGCTCAGCTGGAAATGGAAGATGAGGACACCATCGACGTG
TTCCAGCAGCAGACCcatcatcatcatcatcactaagcggccgc

Amino Acid Sequence

MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGA
FRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATK
TDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEK
RPVDGGSGGGSGGGSGGGSGGGSENLYFQGMADEKPKEGV*TENNDHINL
KVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINET
DTPAQLEMEDEDTIDVFQQQTHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65585
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone produced by Virdee Lab. Entire fusion synthesised (Nco1)UBE2L3-SUMO2(TAG11)-6His(Not1).; Entire cassette codon optimised (Human); Small ubiquitin-related modifier 2 [Homo sapiens] lso a aa 1-91 NP_008868.3
• Low Temp: No