cDNA Clone UBE2D3

• Plasmid: pET156-UBE2D3-SUMO2 (TAG11)
• Parent Plasmid: pET156
• Expressed: UBE2D3-SUMO2 (TAG11)-6His
• Genbank: NA
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ccATGGCCTTGAAGAGAATTAACAAGGAGCTGAGCGATCTGGCTAGGGATCCACCCGCCCAGTGTAGCGCTGGCCCAGTGGGCGATGACATGTTCCACTGGCAGGCCACCATCATGGGGCCCAACGATTCCCCCTACCAGGGCGGGGTGTTCTTCCTGACCATCCACTTCCCCACAGACTACCCCTTCAAGCCCCCCAAGGTGGCCTTCACCACAAGGATCTACCACCCCAACATCAACAGCAACGGGAGCATCTGCCTGGACATCCTGAGGAGCCAGTGGAGCCCCGCCCTGACAATCTCCAAGGTGCTGCTGAGCATCTGCAGCCTGCTGTGCGACCCCAACCCCGATGACCCCCTGGTGCCCGAGATCGCCCGGATCTACAAGACCGACCGGGATAAGTACAACAGGATCAGCAGGGAGTGGACACAGAAGTACGCTATGGGCGGGAGCGGGGGCGGGTCCGGGGGCGGGAGCGGCGGCGGCAGCGGGGGGGGCTCCGAGAACCTGTACTTTCAAGGAATGGCAGATGAGAAGCCCAAAGAGGGCGTGTAGACAGAGAACAACGACCACATCAACCTGAAGGTGGCCGGCCAGGATGGGAGCGTGGTGCAGTTCAAGATCAAGAGGCACACCCCCCTGAGCAAGTTGATGAAGGCCTACTGCGAGAGGCAGGGCCTGTCCATGAGGCAGATCCGGTTCCGGTTCGATGGGCAGCCCATCAACGAGACAGATACACCCGCCCAGCTGGAGATGGAGGATGAAGACACAATCGATGTGTTTCAGCAGCAGACCCACCACCACCATCACCACTGAgcggccgc

Amino Acid Sequence

MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAMGGSGGGSGGGSGGGSGGGSENLYFQGMADEKPKEGV*TENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET156-UBE2D3-SUMO2 (TAG11)188.82 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65637
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Entire fusion synthesised (Nco1)UBE2D3-SUMO2(TAG11)-6His(Not1) and sub cloned into the Nco-Not backbone of 65585.; Entire cassette codon optimised (Human); Small ubiquitin-related modifier 2 [Homo sapiens] lso a aa 1-91 NP_008868.3
• Low Temp: No