cDNA Clone PPM1H R338A

• Plasmid: pET15b 6HIS SUMO PPM1H R338A
• Parent Plasmid: pET5b 6HIS SUMO
• Expressed: 6HIS SUMO PPM1H R338A
• Genbank: Q9ULR3.2
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ATGCTCACTCGAGTGAAATCTGCCGTGGCCAATTTCATGGGCGGCATCATGGCTGGCAGCTCAGGCTCCGAGCACGGCGGCGGCAGCTGCGGAGGCTCGGACCTGCCCCTGCGTTTCCCCTACGGGCGGCCAGAGTTCCTGGGGCTGTCTCAGGACGAGGTGGAGTGCAGCGCCGACCACATCGCCCGCCCCATCCTCATCCTCAAGGAGACTCGGCGGCTGCCCTGGGCCACTGGCTACGCAGAGGTTATCAATGCCGGGAAGAGCACACACAATGAAGACCAAGCCAGCTGTGAGGTGCTCACTGTGAAGAAGAAGGCAGGGGCCGTGACCTCAACCCCAAACAGGAACTCATCCAAGAGACGGTCCTCCCTTCCCAATGGGGAAGGGCTGCAGCTGAAGGAGAACTCGGAATCCGAGGGTGTTTCCTGCCACTATTGGTCGCTGTTTGACGGGCACGCGGGGTCCGGGGCCGCGGTGGTGGCGTCACGCCTGCTGCAGCACCACATCACGGAGCAGCTGCAGGACATCGTGGACATCCTGAAGAACTCCGCCGTCCTGCCCCCTACCTGCCTGGGGGAGGAGCCTGAGAACACGCCCGCCAACAGCCGGACTCTGACCCGGGCAGCCTCCCTGCGCGGAGGGGTGGGGGCCCCGGGCTCCCCCAGCACGCCCCCCACACGCTTCTTTACCGAGAAGAAGATTCCCCATGAGTGCCTGGTCATCGGAGCGCTTGAAAGTGCATTCAAGGAAATGGACCTACAGATAGAACGAGAGAGGAGTTCATATAATATATCTGGTGGCTGCACGGCCCTCATTGTGATTTGCCTTTTGGGGAAGCTGTATGTTGCAAATGCTGGGGATAGCAGGGCCATAATCATCAGAAATGGAGAAATTATCCCCATGTCTTCAGAATTTACCCCCGAGACGGAGCGCCAGCGACTTCAGTACCTGGCATTCATGCAGCCTCACTTGCTGGGAAATGAGTTCACACATTTGGAGTTTCCAAGGgcAGTACAGAGAAAGGAGCTTGGAAAGAAGATGCTCTACAGGGACTTTAATATGACAGGCTGGGCATACAAAACCATTGAGGATGAGGACTTGAAGTTCCCCCTTATATATGGAGAAGGCAAGAAGGCCCGGGTAATGGCAACTATTGGAGTGACCAGGGGACTTGGGGACCATGACCTGAAGGTGCATGACTCCAACATCTACATTAAACCATTCCTGTCTTCAGCTCCAGAGGTAAGAATCTACGATCTTTCAAAATATGATCATGGATCAGATGATGTGCTGATCTTGGCCACTGATGGACTCTGGGACGTTTTATCAAATGAAGAAGTAGCAGAAGCAATCACTCAGTTTCTTCCTAACTGTGATCCAGATGATCCTCACAGGTACACACTGGCAGCTCAGGACCTGGTGATGCGTGCCCGGGGTGTGCTGAAGGACAGAGGATGGCGGATATCTAATGACCGACTGGGCTCAGGAGACGACATTTCTGTATATGTCATTCCTTTAATACATGGAAACAAGCTGTCATGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQA
SCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEP
ENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRN
GEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRAVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHD
LKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGS
GDDISVYVIPLIHGNKLS*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b 6HIS SUMO PPM1H R338A288.46 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU68554
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker after cleavage deleted making clone flush
• Low Temp: No