cDNA Clone MARCH6

• Plasmid: pEX-GST-MARCH6 Met1-Ile90 W43A
• Parent Plasmid: pEX-GST
• Expressed: MARCH6 Met1-Ile90 W43A -GST
• Genbank: NM_005885
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGACACCGCGGAGGAAGACATATGTAGAGTGTGTCGGTCAGAAGGAACACCTGAGAAACCGCTTTATCATCCTTGTGTATGTACTGGCAGTATTAAGTTTATCCATCAAGAATGCTTAGTTCAAgcGCTGAAACACAGTCGAAAAGAATACTGTGAATTATGCAAGCACAGATTTGCTTTTACACCAATTTATTCTCCAGATATGCCTTCACGGCTTCCAATTCAAGACATATTTGCTGGACTGGTTACAAGTATTGGCACTGCAATAgcggccgc

Amino Acid Sequence

MGSMDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQALKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIAAALEVLFQGPLSRMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pEX-GST-MARCH6 Met1-Ile90 W43A221.59 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65738
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 30890; Clone carries N-terminal 3aa extension MGS upstream of native methionine (derived from parental vector)
• Low Temp: No