cDNA Clone tim-1 ubc-3 ubc-7 (worm)

• Plasmid: wRp-315 [RNAi of tim-1 ubc-3 ubc-7]
• Parent Plasmid: DU70356
• Genbank: -

Sequence of Insert

see snapgene file

Amino Acid Sequence

see snapgene file

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: wRp-315 [RNAi of tim-1 ubc-3 ubc-7] 32.05 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU70391
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone made and deposited by Karim Labib lab. 1kb of tim-1 was amplified from cDNA library, 5 end with XbaI site and 3 end with SpeI site.. Then inserted into SpeI site on L4440 (DU70356). ubc-3 ubc-7 were amplified from cDNA library then assembled into one fragment by PCR, both ends with SpeI sites. Then inserted into SpeI site on the previous tim-1 RNAi plasmid.
• Low Temp: No