cDNA Clone SARS-CoV2 (2019-nCoV) Nsp5

• Plasmid: pRF063 (14His-SUMO-Nsp5)
• Parent Plasmid: K27SUMO (see notes)
• Expressed: 14His-SUMO-Nsp5
• Genbank: MN908947.3
• Species: SARS-CoV-2

Sequence of Insert

see snapgene file

Amino Acid Sequence

MSKHHHHSGHHHTGHHHHSGSHHHTGSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQAD
QTPEDLDMEDNDIIEAHREQIGGSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRV
IGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDL
EGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMN
GRTILGSALLEDEFTPFDVVRQCSGVTFQ*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pRF063 (14His-SUMO-Nsp5)37.63 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU70486
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone made and deposited by Karim Labib lab. ; Backbone plasmid: K27SUMO (https://www.sciencedirect.com/science/article/pii/S009286741401040X)
• Low Temp: No