Synonyms
R1 starch-granule-bound R1 protein , alpha-glucan water dikinase
Plasmid
pSc-B-GWD variant
Parent Plasmid
pSC-B
Expressed
GST-GWD variant
Genbank
Gene ID: 102577510 NM_001288123.1 → NP_001275052.1
Vector Type
Other
Antibiotic
Amp; Kan
Full Snapgene Map
pSc-B-GWD variant2.28 MB
Snapgene files can be opened using software available here: www.snapgene.com
DU Number
DU65961
Unit Source
Location
PPU
MTA
Notes
R1 gene from Solanum tuberosum (potato). N-term GST for bacterial expression.
Chloroplastic. total RNA extracted from 3 week old Desiree (v) leaves.
Silent changes compared to NCBI ref at:
Pro46 (CCA>CCT), Gly135 (GGG>GGA), Leu420 (CTA>CTT), Ser1011 (AGC>AGT), Ala1165 (GCT>GCA), Cys1293 (TGT>TGC), Tyr1339 (TAT>TAC), Asp1412 (GAT>GAC)
non silent at E66G, K68N, R95G, L447I, A599T, E1034K, A1312T, T1356P, S1427N
Asn inserted between Ile381 and Lys382 (all numbers here after Ile381 are cited according to the NCBI ref but will be +1 for this variant)
1146bp Deletion spanning Glu625 - Ser1006
Chloroplastic. total RNA extracted from 3 week old Desiree (v) leaves.
Silent changes compared to NCBI ref at:
Pro46 (CCA>CCT), Gly135 (GGG>GGA), Leu420 (CTA>CTT), Ser1011 (AGC>AGT), Ala1165 (GCT>GCA), Cys1293 (TGT>TGC), Tyr1339 (TAT>TAC), Asp1412 (GAT>GAC)
non silent at E66G, K68N, R95G, L447I, A599T, E1034K, A1312T, T1356P, S1427N
Asn inserted between Ile381 and Lys382 (all numbers here after Ile381 are cited according to the NCBI ref but will be +1 for this variant)
1146bp Deletion spanning Glu625 - Ser1006
Low Temp
No
Price
£125.00
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