cDNA Clone Human POLE2/POLE3/POLE4

• Synonyms: DPB2, DPB3, DPB4
• Plasmid: pBIG1b Human POLE2_Strep-Tev-POLE3_POLE4 (pTL12)
• Parent Plasmid: pBIG1b
• Expressed: Human POLE2_Strep-Tev-POLE3_POLE4
• Genbank: NM_002692.4 for POLE2; ENST00000374169.7 for POLE3; NM_019896.4 for POLE4
• Tag: StrepII
• Species: Human

Sequence of Insert

see snapgene file

Amino Acid Sequence

see snapgene file

• Vector Type: Baculovirus
• Antibiotic: Spectinomycin
• Full Snapgene Map: pBIG1b Human POLE2_Strep-Tev-POLE3_POLE4 (pTL12)60.55 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU70589
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Gibson assembly into pBIG1b followed Weissmann, Petzold et al., 2016. Clone deposited by Thanh Le (Karim Labib group, MRCPPU)
• Low Temp: No