cDNA Clone PPM1H/J chimera

• Plasmid: pMEX3Cb PPM1H - J flap chimera
• Parent Plasmid: pMEX3Cb
• Expressed: MBP 3C PPM1H - J flap chimera
• Genbank: -
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCAGCGATCTGCCGCTGCGCTTTCCGTATGGCCGCCCGGAATTTCTGGGCCTGAGCCAGGATGAAGTGGAATGCAGCGCGGATCATATTGCGCGCCCGATTCTGATTCTGAAAGAAACCCGCCGCCTGCCGTGGGCGACCGGCTATGCGGAAGTGATTAACGCGGGCAAAAGCACCCATAACGAAGATCAGGCGAGCTGCGAAGTGCTGACCGTGAAAAAAAAAGCGGGCGCGGTGACCAGCACCCCGAACCGCAACAGCAGCAAACGCCGCAGCAGCCTGCCGAACGGCGAAGGCCTGCAGCTGAAAGAAAACAGCGAAAGCGAAGGCGTGAGCTGCCATTATTGGAGCCTGTTTGATGGCCATGCGGGCAGCGGCGCGGCGGTGGTGGCGAGCCGCCTGCTGCAGCATCATATTACCGAACAGCTGCAGGATATTGTGGATATTCTGAAAAACAGCGCGGTGCTGCCGCCGACCTGCCTGGGCGAAGAACCGGAAAACACCCCGGCGAACAGCCGCACCCTGACCCGCGCGGCGAGCCTGCGCGGCGGCGTGGGCGCGCCGGGCAGCCCGAGCACCCCGCCGACCCGCTTTTTTACCGAAAAAAAAATTCCGCATGAATGCCTGGTGATTGGCGCGCTGGAAAGCGCGTTTAAAGAAATGGATCTGCAGATTGAACGCGAACGCAGCAGCTATAACATTAGCGGCGGCTGCACCGCGCTGATTGTGATTTGCCTGCTGGGCAAACTGTATGTGGCGAACGCGGGCGATAGCCGCGCGATTATTATTCGCAACGGCGAAATTATTCCGATGAGCCGCGAATTTACCCCGGAAACCGAACGCCAGCGCCTGCAGCTGCTGGGCTTTCTGAAACCGGAACTGCTGGGCAGCGAATTTACCCATCTGGAATTTCCGCGCCGCGTGCTGCCGAAAGAACTGGGCCAGCGCATGCTGTATCGCGATCAGAACATGACCGGCTGGGCGTATAAAAAAATTGAACTGGAAGATCTGCGCTTTCCGCTGGTGTGCGGCGAAGGCAAAAAAGCGCGCGTGATGGCGACCATTGGCGTGACCCGCGGCCTGGGCGATCATAGCCTGAAAGTGTGCAGCAGCACCCTGCCGATTAAACCGTTTCTGAGCTGCTTTCCGGAAGTGCGCATTTATGATCTGAGCAAATATGATCATGGCAGCGATGATGTGCTGATTCTGGCGACCGATGGCCTGTGGGATGTGCTGAGCAACGAAGAAGTGGCGGAAGCGATTACCCAGTTTCTGCCGAACTGCGATCCGGATGATCCGCATCGCTATACCCTGGCGGCGCAGGATCTGGTGATGCGCGCGCGCGGCGTGCTGAAAGATCGCGGCTGGCGCATTAGCAACGATCGCCTGGGCAGCGGCGATGATATTAGCGTGTATGTGATTCCGCTGATTCATGGCAACAAACTGAGCtgaGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
LGSSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGE
GLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTRF
FTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSREFTPETERQRLQLLGFLKPELLGSE
FTHLEFPRRVLPKELGQRMLYRDQNMTGWAYKKIELEDLRFPLVCGEGKKARVMATIGVTRGLGDHSLKVCSSTLPIKPFLSCFPEVRIYDLSKYDHGSD
DVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pMEX3Cb PPM1H - J flap chimera115.47 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU70608
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No