cDNA Clone HOIL1

• Plasmid: pET156His SUMO-HOIL [D233-H510] R239A E243A
• Parent Plasmid: pET156His SUMO
• Expressed: His-SUMO-HOIL1 [D233-H510] R239A E243A
• Genbank: NM_031229
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GCGGCCGCGGACGAGGAGGAGCGAGCGgcCCTGGCGGGCGcGGAGGAGGCGCTGCGTCAGTACCAGCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCAGCACGTCCAGCTGGACCAGAGGAGCCTGGTGCTGAACACGGAGCCCGCCGAGTGCCCCGTGTGCTACTCGGTGCTGGCGCCCGGCGAGGCCGTGGTGCTGCGTGAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGGCACCATCCGCAACAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACTCGTGCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCTGAGGATTACCAGCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCGCAGTGCCTTCAGCTACCATTGCAAGACCCCAGATTGCAAGGGATGGTGCTTCTTTGAGGATGATGTCAATGAGTTCACCTGCCCTGTGTGTTTCCACGTCAACTGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTGCAAGGAGTATCAGGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGACGACAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGCCCCCAGTGCCAGATCGTGGTACAGAAGAAGGACGGCTGCGACTGGATCCGCTGCACCGTCTGCCACACCGAGATCTGCTGGGTCACCAAGGGCCCACGCTGGGGCCCTGGGGGCCCAGGAGACACCAGCGGGGGCTGCCGCTGCAGGGTAAATGGGATTCCTTGCCACCCAAGCTGTCAGAACTGCCACTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVDGAAADEEERAALAGAEEALRQYQQRKQQQQEGNYLQHVQ
LDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCRECLQGTIRNSQ
EAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGISIAENRSAF
SYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQMNCKEYQED
LALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKDGCDWIRCTV
CHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQNCH*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET156His SUMO-HOIL [D233-H510] R239A E243A199.06 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65986
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 45322; SUMO GG not flush with gene ORF (extraneous residues SGEFGVDGAAA)
• Low Temp: No