cDNA Clone GWD (potato R1)

• Synonyms: R1 starch-granule-bound R1 protein , alpha-glucan water dikinase
• Plasmid: pGEX6P-1-GWD variant
• Parent Plasmid: pGEX6P-1
• Expressed: GST-GWD variant
• Genbank: Gene ID: 102577510 NM_001288123.1 → NP_001275052.1
• Tag: GST

Sequence of Insert

ggatccatgagtaattccttagggaataacttgctgtaccagggattcctaacctcaacagtgttggaacataaaagtagaatcagtcctccttgtgttggaggcaattctttgtttcaacaacaagtgatctcgaaatcacctttatcaactgagtttcgaggtaacaggttaaaggtgcagaaaaagaaaatacctatgggaaagaaccgtgctttttctagttctcctcatgctgtacttaccactgatacctcttctgagctagcagaaaagttcagtctagaagggaatattgagctacaggttgatgttaggcctcccacttcaggtgatgtgtcctttgtggattttcaagctacaaatggtagtgataaactgtttttgcactggggggcagtaaagttcggaaaagaaacatggtctcttcctaatgatcgtccagatgggaccaaagtgtacaagaacaaagcacttagaactccatttgttaaatctggctctaactccatcctgagactggagatacgggacactgctatcgaagctattgagtttctcatatacgatgaagcctacgataaatggataaagaataatggtggcaattttcgtgtcaaattgtcaagaaaagagatacgaggcccagatgtttcagttcctgaggagcttgtacagatccaatcatatttgaggtgggagaggaagggaaaacagaattacacccctgagaaagagaaggaggaatatgaggctgctcgaactgagctacaggaggaaatagctcgtggtgcttccatacaggacattcgagcaaggctaacaaaaactaatgataaaagtcaaagcaaagaagagcctcttcatgtaacaaagagtgaaatacctgatgaccttgcccaagcacaagcttacattaggtgggagaaagcaggaaagccgaactatcctccagaaaagcaaattgaagaactcgaagaagcaagaagagaattgcaacttgagcttgagaaaggcattacccttgatgagttgcggaaaaagattacaaaaggggagataaaaactaaggcggaaaagcacgtgaaaagaagctcttttgccgttgaaagaatccaaagaaagaagagagactttgggcagcttattaataagtatccttccagtcctgcagtacaagtacaaaaggtcttggaagaaccaccagccttatctaaaattaagctgtatgccaaggagaaggaggagcagattgatgatccgatccttaataaaaagatctttaaggtcgatgatggggagctactggtactggtagcaaagtcctctgggaagacaaaagtacatatagctacagatctgaatcagccaattactcttcactgggcattatccaaaagtcgtggagagtggatggtaccaccttcaagcatattgcctcctggatcaattattttagacaaggctgccgaaacacctttttccgccagttcttctgatggtctaacttctaaggtacaatctttggatatagtaattgaagatggcaattttgtggggatgccatttgttcttttgtctggtgaaaaatggattaagaaccaagggtcggatttctatgttgacttcagtgctgcatccaaattagcactcaaggctgctggggatggcagtggaactgcaaagtctttactggataaaatagcagatatggaaagtgaggctcagaagtcatttatgcaccggtttaatattgctgctgacttgatagaagatgccactagtgctggtgaacttggttttactggaattcttgtatggatgaggttcatggctacaaggcaactgatatggaacaaaaactataacgtaaaaccacgttggcagattatcagtccagttgaagccgttggatatgttgtcgttgtggatgagttgctttcagttcagaatgaaatctacaagaagcccacgatcttagtagcaaactctgttaaaggagaggaggaaattcctgatggtgctgttgccctgataacaccagacatgccagatgttctttcacatgtttctgttcgagctagaaatgggaaggtttgctttgctacatgctttgatcccaatatattggctgacctccaagcaaaggaaggaaggattttgctcttaaagcctacaccttcagacataatctatagtgaggtgaatgagattgagctccaaagttcaagtaacttggtagaagctgaaacttcagcaacacttagattggtgaaaaagcaatttggtggttgttacgcaatatcagcagatgaattcacaagtgaaatggttggagctaaatcacgtaatattgcatatctgaaaggaaaagtgccttcctcggtgggaattcctacgtcagtagctcttccatttggagtctttgagaaagtactttcagacgacataaatcagggagtggcaaaagagttgcaaattctgacgaaaaaactatctgaaggagacttcagcgctcttggtgaaattcgcacaacgattttagatctttcagcaccagctcaattggtcaaagagctgaaggaaaagatgcagggttctggcatgccttggcctggtgatgaaggtccaaagcggtgggaacaagcatggatggccataaaaaaggtgtgggcttcaaaatggaatgagagagcatacttcagcacaaggaaggtgaaactggatcatgactatctgtgcatggctgtccttgttcaagaaataataaatgctgattatgcatttgtcattcacacaaccaacccatcttccggagacgactcagaaatatatgccgaggtggtcaggggccttggggaaacacttgttggagcttacccaggacgtgctttgagttttatctgcaagaaaaaggatctcaactctCctcaagtgttaggttacccaagcaaaccgatcggccttttcataaaaagatctatcatcttccgatctgattccaatggggaagatttggaaggttatgccggtgctggcctctacgacagtgtaccaatggatgaggaggaaaaagttgtaattgattactcttccgacccattgataactgatggtaacttccgccagacaatcctgtccaacattgctcgtgctggacatgctatcgaggagctatatggctctcctcaagacatcgagggtgtagtgagggatggaaagatttatgtcgttcagacaagacctcagatgtgagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSNSLGNNLLYQGFLTSTV
LEHKSRISPPCVGGNSLFQQQVISKSPLSTEFRGNRLKVQKKKIPMGKNR
AFSSSPHAVLTTDTSSELAEKFSLEGNIELQVDVRPPTSGDVSFVDFQAT
NGSDKLFLHWGAVKFGKETWSLPNDRPDGTKVYKNKALRTPFVKSGSNSI
LRLEIRDTAIEAIEFLIYDEAYDKWIKNNGGNFRVKLSRKEIRGPDVSVP
EELVQIQSYLRWERKGKQNYTPEKEKEEYEAARTELQEEIARGASIQDIR
ARLTKTNDKSQSKEEPLHVTKSEIPDDLAQAQAYIRWEKAGKPNYPPEKQ
IEELEEARRELQLELEKGITLDELRKKITKGEIKTKAEKHVKRSSFAVER
IQRKKRDFGQLINKYPSSPAVQVQKVLEEPPALSKIKLYAKEKEEQIDDP
ILNKKIFKVDDGELLVLVAKSSGKTKVHIATDLNQPITLHWALSKSRGEW
MVPPSSILPPGSIILDKAAETPFSASSSDGLTSKVQSLDIVIEDGNFVGM
PFVLLSGEKWIKNQGSDFYVDFSAASKLALKAAGDGSGTAKSLLDKIADM
ESEAQKSFMHRFNIAADLIEDATSAGELGFTGILVWMRFMATRQLIWNKN
YNVKPRWQIISPVEAVGYVVVVDELLSVQNEIYKKPTILVANSVKGEEEI
PDGAVALITPDMPDVLSHVSVRARNGKVCFATCFDPNILADLQAKEGRIL
LLKPTPSDIIYSEVNEIELQSSSNLVEAETSATLRLVKKQFGGCYAISAD
EFTSEMVGAKSRNIAYLKGKVPSSVGIPTSVALPFGVFEKVLSDDINQGV
AKELQILTKKLSEGDFSALGEIRTTILDLSAPAQLVKELKEKMQGSGMPW
PGDEGPKRWEQAWMAIKKVWASKWNERAYFSTRKVKLDHDYLCMAVLVQE
IINADYAFVIHTTNPSSGDDSEIYAEVVRGLGETLVGAYPGRALSFICKK
KDLNSPQVLGYPSKPIGLFIKRSIIFRSDSNGEDLEGYAGAGLYDSVPMD
EEEKVVIDYSSDPLITDGNFRQTILSNIARAGHAIEELYGSPQDIEGVVR
DGKIYVVQTRPQM*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-GWD variant106.57 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65991
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: R1 gene from Solanum tuberosum (potato). N-term GST for bacterial expression.; Chloroplastic. total RNA extracted from 3 week old Desiree (v) leaves.; Silent changes compared to NCBI ref at: ; Pro46 (CCA>CCT), Gly135 (GGG>GGA), Leu420 (CTA>CTT), Ser1011 (AGC>AGT), Ala1165 (GCT>GCA), Cys1293 (TGT>TGC), Tyr1339 (TAT>TAC), Asp1412 (GAT>GAC); ; non silent at E66G, K68N, R95G, L447I, A599T, E1034K, A1312T, T1356P, S1427N; ; Asn inserted between Ile381 and Lys382 (all numbers here after Ile381 are cited according to the NCBI ref but will be +1 for this variant); 1146bp Deletion spanning Glu625 - Ser1006
• Low Temp: No