cDNA Clone TIFA

• Plasmid: pcDNA5D FRT TO (UbC Pr)-FLAG-TIFA
• Parent Plasmid: pcDNA5D FRT TO (UbC Pr)
• Expressed: FLAG-TIFA[WT]
• Genbank: -
• Tag: FLAG
• Species: Human

Sequence of Insert

cttaagcttGCCACCATGGACTACAAGGACGATGACGATAAGATGACCAGTTTTGAAGATGCTGACACAGAAGAGACAGTAACTTGTCTCCAGATGACGGTTTACCATCCTGGCCAGTTGCAGTGTGGAATATTTCAGTCAATAAGTTTTAACAGAGAGAAACTCCCTTCCAGCGAAGTGGTGAAATTTGGCCGAAATTCCAACATCTGTCATTATACTTTTCAGGACAAACAGGTTTCCCGAGTTCAGTTTTCTCTGCAGCTGTTTAAAAAATTCAACAGCTCAGTTCTCTCCTTTGAAATAAAAAATATGAGTAAAAAGACCAATCTGATCGTGGACAGCAGAGAGCTGGGCTACCTAAATAAAATGGACCTGCCATACAGGTGCATGGTCAGATTCGGAGAGTATCAGTTTCTGATGGAGAAGGAAGATGGCGAGTCATTGGAATTTTTTGAGACTCAATTTATTTTATCTCCAAGATCACTCTTGCAAGAAAACAACTGGCCACCACACAGGCCCATACCGGAGTATGGCACTTACTCGCTCTGCTCCTCCCAAAGCAGTTCTCCGACAGAAATGGATGAAAATGAGTCATGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKMTSFEDADTEETVTCLQMTVYHPGQLQCGIFQSISFNREKLPSSEVVKFGRNSNICHYTFQDKQVSRVQFSLQLFKKFNSSVLSFEIKNMSKKTNLIVDSRELGYLNKMDLPYRCMVRFGEYQFLMEKEDGESLEFFETQFILSPRSLLQENNWPPHRPIPEYGTYSLCSSQSSSPTEMDENES*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5D FRT TO (UbC Pr)-FLAG-TIFA272.44 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU71017
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 70173 (pcDNA5D FRT TO Ubc Pr) 3HA tag removed by cloning FLAG-TIFA into the upstream AflII site
• Low Temp: No