cDNA Clone Fbx045

• Plasmid: pOPc-Lac-GST-MYCBP2 Lys2458-Asp2553; 6His-SUMO-Fbx045
• Parent Plasmid: pOPc-GST with Lac Operon restored
• Expressed: 6His-SUMO-Fbx045
• Genbank: NM_015057.4
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCAAATAATTTTGTTTAACTTTAAGAAGGAGATATATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCAACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGTCATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAACTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAACAAACGGGGGGAATGGCGGCGCCGGCCCCGGGGGCTGGGGCAGCCTCGGGCGGCGCTGGCTGTAGCGGCGGCGGCGCGGGCGCGGGCGCGGGCTCGGGCTCTGGGGCCGCGGGGGCCGGGGGCCGGCTGCCCAGCCGGGTGCTGGAGTTGGTGTTCTCTTACCTGGAGCTGTCCGAGCTGCGGAGCTGCGCCCTGGTGTGCAAGCACTGGTATCGCTGCCTGCACGGCGATGAGAACAGCGAGGTGTGGCGGAGCCTGTGCGCCCGCAGCCTGGCAGAAGAGGCTCTGCGCACGGACATCCTGTGCAACCTGCCCAGCTACAAGGCCAAGATACGTGCTTTTCAACATGCCTTCAGCACTAATGACTGCTCCAGGAATGTCTACATTAAGAAGAATGGCTTTACTTTACATCGAAACCCCATTGCTCAGAGCACTGATGGTGCAAGGACCAAGATTGGTTTCAGTGAGGGCCGCCATGCATGGGAAGTGTGGTGGGAGGGCCCTCTGGGCACTGTGGCAGTGATTGGAATTGCCACAAAACGGGCCCCCATGCAGTGCCAAGGTTATGTGGCATTGCTGGGCAGTGATGACCAGAGCTGGGGCTGGAATCTGGTGGACAATAATCTACTACATAATGGAGAAGTCAATGGCAGTTTTCCACAGTGCAACAACGCACCAAAATATCAGATAGGAGAAAGGATTCGAGTCATCTTGGACATGGAAGATAAGACTTTAGCTTTTGAACGTGGATATGAGTTCCTGGGGGTTGCTTTTAGAGGACTTCCAAAGGTCTGCTTATACCCAGCAGTTTCTGCTGTATATGGCAACACAGAAGTGACTTTGGTTTACCTTGGAAAACCTTTGGACGGATGAaagctt

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMAAPAPGAGAASGGAGCSGGGAGAGAGSGSGAAGAGGRLPSRVLELVFSYLELSELRSCALVCKHWYRCLHGDENSEVWRSLCARSLAEEALRTDILCNLPSYKAKIRAFQHAFSTNDCSRNVYIKKNGFTLHRNPIAQSTDGARTKIGFSEGRHAWEVWWEGPLGTVAVIGIATKRAPMQCQGYVALLGSDDQSWGWNLVDNNLLHNGEVNGSFPQCNNAPKYQIGERIRVILDMEDKTLAFERGYEFLGVAFRGLPKVCLYPAVSAVYGNTEVTLVYLGKPLDG*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pOPc-Lac-GST-MYCBP2 Lys2458-Asp2553; 6His-SUMO-Fbx0451.04 MBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU71133
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: EcoR1/HindIII flanked pOPc leader-6His-SUMO-Fbxo45 amplified from 65322 derived Fbxo45 sequence with the following silent changes made (Arg229 (AGA>AGG) with NW7752/53 to kill native EcoR1, Tyr67 (TAC>TAT) with NW7756/57 to kill native Kpn1). Subcloned into EcoR1-HindIII backbone of 71107
• Low Temp: No