Synonyms
TNF receptor-associated factor 6
Plasmid
pcDNA5 FRT/TO.PURO.FLAG.DU TRAF6 KallR
Parent Plasmid
pcDNA5 FRT/TO.PURO.FLAG.DU
Expressed
human FLAG TRAF6 KallR
Genbank
NM_145803.3 → NP_665802.1
Species
Human
Sequence of Insert
View Sequence
ggatccATGAGTCTGCTAAACTGTGAAAACAGCTGTGGATctAGCCAGTCTGAAAGTGACTGCTGTGTGGCCATGGCCAGCTCCTGTAGCGCTGTAACAAgAGATGATAGTGTGGGTGGAACTGCCAGCACGGGGAACCTCTCCAGCTCATTTATGGAGGAGATCCAGGGATATGATGTAGAGTTTGACCCACCCCTGGAAAGCAgGTATGAATGCCCCATCTGCTTGATGGCATTACGAGAAGCAGTGCAAACGCCATGCGGCCATAGGTTCTGCAgAGCCTGCATCATAAgATCAATAAGGGATGCAGGTCACAgATGTCCAGTTGACAATGAAATACTGCTGGAAAATCAACTATTTCCAGACAATTTTGCAAgACGTGAGATTCTTTCTCTGATGGTGAgATGTCCAAATGAAGGTTGTTTGCACAgGATGGAACTGAGACATCTTGAGGATCATCAAGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCAATGCCAGCGTCCCTTCCAAAgATTCCATATTAATATTCACATTCTGAgGGATTGTCCAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCATTTGAAGATAgAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCATCTGTGAATACTGCAATACTATACTCATCAGAGAACAGATGCCTAATCATTATGATCTAGACTGCCCTACAGCCCCAATTCCATGCACATTCAGTACTTTTGGTTGCCATGAAAgGATGCAGAGGAATCACTTGGCACGCCACCTACAAGAGAACACCCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAGTTTGAGCGTTATACCCGACTCTGGGTATATCTCAGAGGTCCGGAATTTCCAGGAAACTATTCACCAGTTAGAGGGTCGCCTTGTAAGACAAGACCATCAAATCCGGGAGCTGACTGCTAgAATGGAAACTCAGAGTATGTATGTAAGTGAGCTCAgACGAACCATTCGAACCCTTGAGGACAgAGTTGCTGAAATCGAAGCACAGCAGTGCAATGGAATTTATATTTGGAgGATTGGCAACTTTGGAATGCATTTGAgATGTCAAGAAGAGGAGAgACCTGTTGTGATTCATAGCCCTGGATTCTACACTGGCAgACCCGGGTACAgACTGTGCATGCGCTTGCACCTTCAGTTACCGACTGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAATGCAAGGAGAATATGACAGCCACCTCCCTTGGCCCTTCCAGGGTACAATACGCCTTACAATTCTTGATCAGTCTGAAGCACCTGTAAGGCAAAACCACGAAGAGATAATGGATGCCAgACCAGAGCTGCTTGCTTTCCAGCGACCCACAATCCCACGGAACCCAAgAGGTTTTGGCTATGTAACTTTTATGCATCTGGAAGCCCTAAGACAAAGAACTTTCATTAgGGATGACACATTATTAGTGCGCTGTGAGGTCTCCACCCGCTTTGACATGGGTAGCCTTCGGAGGGAGGGTTTTCAGCCACGAAGTACTGATGCAGGGGTATAGGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMSLLNCENSCGSSQSESDCCVAMASSCSAVTRDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESRYECPICLMALREAVQTPCGHRFCRACIIRSIRDAGHRCPVDNEILLENQLFPDNFARREILSLMVRCPNEGCLHRMELRHLEDHQAHCEFALMDCPQCQRPFQRFHINIHILRDCPRRQVSCDNCAASMAFEDREIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHERMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTARMETQSMYVSELRRTIRTLEDRVAEIEAQQCNGIYIWRIGNFGMHLRCQEEERPVVIHSPGFYTGRPGYRLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDARPELLAFQRPTIPRNPRGFGYVTFMHLEALRQRTFIRDDTLLVRCEVSTRFDMGSLRREGFQPRSTDAGV*
Vector Type
Mammalian
Antibiotic
Amp
Full Snapgene Map
pcDNA5 FRT/TO.PURO.FLAG.DU TRAF6 KallR105.46 KB
Snapgene files can be opened using software available here: www.snapgene.com
DU Number
DU71214
Unit Source
Location
PPU
MTA
Notes
Parental 45940
Ser12 TCC>TCT native internal BamHI site killed
23 Lysines mutated to Arginines:
K32, K67, K91, K96, K104, K124, K133, K142, K171, K180, K201, K252, K319, K331, K340, K356, K365, K371, K384, K388, K453, K469, K489
Ser12 TCC>TCT native internal BamHI site killed
23 Lysines mutated to Arginines:
K32, K67, K91, K96, K104, K124, K133, K142, K171, K180, K201, K252, K319, K331, K340, K356, K365, K371, K384, K388, K453, K469, K489
Low Temp
No
Price
£125.00
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