cDNA Clone SV2A

• Plasmid: pCMVmod SV2A pHluorin (intra-luminal)
• Parent Plasmid: pCMV mod
• Expressed: SV2A pHluorin (intra-luminal)
• Genbank: P21579
• Tag: pHluorin (intra-luminal)
• Species: Human

Sequence of Insert

ATGGAAGAGGGCTTCCGAGACCGGGCAGCTTTCATCCGTGGGGCCAAAGA
CATTGCTAAGGAAGTCAAAAAGCATGCGGCCAAGAAGGTGGTGAAGGGCC
TGGACAGAGTCCAGGACGAATATTCCCGAAGATCGTACTCCCGCTTTGAG
GAGGAGGATGATGATGATGACTTCCCTGCTCCCAGTGATGGTTATTACCG
AGGAGAAGGGACCCAGGATGAGGAGGAAGGTGGTGCATCCAGTGATGCTA
CTGAGGGCCATGACGAGGATGATGAGATCTATGAAGGGGAATATCAGGGC
ATTCCCCGGGCAGAGTCTGGGGGCAAAGGCGAGCGGATGGCAGATGGGGC
GCCCCTGGCTGGAGTAAGGGGGGGCTTGAGTGATGGGGAGGGTCCCCCTG
GGGGCCGGGGGGAGGCACAACGACGGAAAGAACGAGAAGAACTGGCCCAA
CAGTATGAAGCCATCCTACGGGAGTGTGGCCACGGCCGCTTCCAGTGGAC
ACTGTATTTTGTGCTTGGTCTGGCGCTGATGGCTGACGGTGTGGAGGTCT
TTGTGGTGGGCTTCGTGCTGCCCAGCGCTGAGAAAGACATGAAAATCCTC
GTGGCGGTGGCGGTCTTTTTTCTCGTTTCCACTCAACTGTTTGCAGAGGA
AATCGGTGCCAACAAGCTTAGTAAAGGAGAAGAACTTTTCACTGGAGTTG
TCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCT
GTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAA
ATTTATTTGCACTACTGGAAAACTACCTGTTCCTTGGCCAACACTTGTCA
CTACTTTAACTTATGGTGTTCAATGCTTTTCAAGATACCCAGATCATATG
AAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGA
AAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAG
TCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATT
GATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTA
TAACGATCACCAGGTGTACATCATGGCAGACAAACAAAAGAATGGAATCA
AAGCTAACTTCAAAATTAGACACAACATTGAAGATGGAGGCGTTCAACTA
GCAGACCATTATCAACAAAATACTCCAATTGGCGATGGGCCCGTCCTTTT
ACCAGACAACCATTACCTGTTTACAACTTCTACTCTTTCGAAAGATCCCA
ACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGG
ATTACACATGGCATGGATGAACTATACAAATGCCTGTCCGACTCCAACAA
AGGCATGCTAGGCCTCATCGTCTACCTGGGCATGATGGTGGGAGCCTTCC
TCTGGGGAGGTCTGGCTGACCGGCTGGGTCGGAGGCAGTGTCTGCTCATC
TCGCTCTCAGTCAACAGCGTCTTCGCCTTCTTCTCATCTTTTGTCCAGGG
TTACGGCACTTTCCTCTTCTGCCGCCTACTTTCTGGGGTTGGGATTGGAG
GGTCCATCCCCATTGTCTTCTCCTATTTCTCCGAGTTTCTGGCCCAGGAG
AAACGAGGGGAGCATTTGAGCTGGCTCTGCATGTTTTGGATGATTGGTGG
CGTGTACGCAGCTGCTATGGCCTGGGCCATCATCCCCCACTATGGGTGGA
GTTTTCAGATGGGTTCTGCCTACCAGTTCCACAGCTGGAGGGTCTTCGTC
CTCGTCTGCGCCTTTCCTTCTGTGTTTGCCATTGGGGCTCTGACCACGCA
GCCTGAGAGCCCCCGTTTCTTCCTAGAGAATGGAAAGCATGATGAGGCCT
GGATGGTGCTGAAGCAGGTCCATGATACCAACATGCGAGCCAAAGGACAT
CCTGAGCGAGTGTTCTCAGTAACCCACATTAAGACGATTCATCAGGAGGA
TGAATTGATTGAGATCCAGTCGGACACAGGGACCTGGTACCAGCGCTGGG
GGGTCCGGGCCTTGAGCCTAGGGGGGCAGGTTTGGGGGAATTTTCTCTCC
TGTTTTGGTCCCGAATATCGGCGCATCACTCTGATGATGATGGGTGTGTG
GTTCACCATGTCATTCAGCTACTATGGCCTGACCGTCTGGTTTCCTGACA
TGATCCGCCATCTCCAGGCAGTGGACTACGCATCCCGCACCAAAGTGTTC
CCCGGGGAGCGCGTAGAGCATGTAACTTTTAACTTCACGTTGGAGAATCA
GATCCACCGAGGCGGGCAGTACTTCAATGACAAGTTCATTGGGCTGCGGC
TCAAGTCAGTGTCCTTTGAGGATTCCCTGTTTGAAGAGTGTTATTTTGAG
GATGTCACATCCAGCAACACGTTTTTCCGCAACTGCACATTCATCAACAC
TGTGTTCTATAACACTGACCTGTTCGAGTACAAGTTTGTGAACAGCCGTC
TGATAAACAGTACATTCCTGCACAACAAGGAGGGCTGCCCGCTAGACGTG
ACAGGGACGGGCGAAGGTGCCTACATGGTATACTTTGTGAGCTTCCTGGG
GACACTGGCAGTGCTTCCTGGGAATATCGTGTCTGCCCTGCTCATGGACA
AGATCGGCAGGCTCAGAATGCTTGCTGGCTCCAGCGTGATGTCCTGTGTC
TCCTGCTTCTTCCTGTCTTTTGGGAACAGTGAGTCGGCCATGATCGCTCT
GCTCTGCCTTTTTGGCGGGGTCAGCATTGCATCCTGGAATGCGCTGGACG
TGTTGACTGTTGAACTCTACCCCTCAGACAAGAGGACCACAGCTTTTGGC
TTCCTGAATGCCCTGTGTAAGCTGGCAGCTGTGCTGGGGATCAGCATCTT
CACATCCTTCGTGGGAATCACCAAGGCTGCACCCATCCTCTTTGCCTCAG
CTGCCCTTGCCCTTGGCAGCTCTCTGGCCCTGAAGCTGCCTGAGACCCGG
GGGCAGGTGCTGCAGTGA

Amino Acid Sequence

MEEGFRDRAAFIRGAKDIAKEVKKHAAKKVVKGLDRVQDEYSRRSYSRFEEEDDDDDFPAPSDGYYRGEGTQDEEEGGASSDATEGHDEDDEIYEGEYQGIPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQRRKEREELAQQYEAILRECGHGRFQWTLYFVLGLALMADGVEVFVVGFVLPSAEKDMKILVAVAVFFLVSTQLFAEEIGANKLSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCLSDSNKGMLGLIVYLGMMVGAFLWGGLADRLGRRQCLLISLSVNSVFAFFSSFVQGYGTFLFCRLLSGVGIGGSIPIVFSYFSEFLAQEKRGEHLSWLCMFWMIGGVYAAAMAWAIIPHYGWSFQMGSAYQFHSWRVFVLVCAFPSVFAIGALTTQPESPRFFLENGKHDEAWMVLKQVHDTNMRAKGHPERVFSVTHIKTIHQEDELIEIQSDTGTWYQRWGVRALSLGGQVWGNFLSCFGPEYRRITLMMMGVWFTMSFSYYGLTVWFPDMIRHLQAVDYASRTKVFPGERVEHVTFNFTLENQIHRGGQYFNDKFIGLRLKSVSFEDSLFEECYFEDVTSSNTFFRNCTFINTVFYNTDLFEYKFVNSRLINSTFLHNKEGCPLDVTGTGEGAYMVYFVSFLGTLAVLPGNIVSALLMDKIGRLRMLAGSSVMSCVSCFFLSFGNSESAMIALLCLFGGVSIASWNALDVLTVELYPSDKRTTAFGFLNALCKLAAVLGISIFTSFVGITKAAPILFASAALALGSSLALKLPETRGQVLQ*

• Vector Type: Mammalian
• Antibiotic: Kan
• Full Snapgene Map: pCMVmod SV2A pHluorin (intra-luminal) 1.3 MB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU42587
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: The vector backbone supplied by Noor Esoof seems to be based on pEGFP-C1.; Prone to recombination.; Vector backbone is best guess from information received. Grow at or below 30ºC
• Low Temp: Yes