cDNA Clone NEDD4.2b1

• Plasmid: pFB Nedd4.2b1 T246A
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-Nedd4.2b1 T246A
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAG
AAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCCATA
TGCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGAC
ATGGAGCATGGATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCA
CCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAG
TGAACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACT
CAGTGGCACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAA
CATCAGACAGATCAACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCA
GGCACATCAGCGAAGACTTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTC
CCCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAATATCGCTGGAGACTC
TCTCGGTCTGGCTCTGCCCCCACCGCCGGCCTCCCCAGGATCTCGGACCA
GCCCTCAGGAGCTGTCAGAGGAACTATGCAGAAGGCTTCAGATCACTCCA
GACTCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTC
AAGGTTGAGGTCATGCAGTGTCACCGACGCAGTTGCAGAACAGGGCCATC
TACCACCGCCCAGTGCCCCAGCTGGGAGAGCGCGTTCATCAGCTGTCACG
GGTGGTGAGGAACCAACGCCATCAGTGGCCTATGTACATACCACGCCGGG
TCTGCCCTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACATACT
ATGTCAATCATAACAATCGAACCACAACTTGGACTCGACCTATCATGCAG
CTTGCAGAAGATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCT
AATCGAGCCTCAGATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAA
CTTTATCTGCCCCGCTGGAGGGTGCCAAGGACTCACCCGTACGTCGGGCT
GTGAAAGACACCCTTTCCAACCCACAGTCCCCACAGCCATCACCTTACAA
CTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCTTGCCGCCCGGCT
GGGAAATGAGGATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAAC
ACAAAGACTACAACCTGGGAAGATCCACGTTTGAAGTTTCCAGTACATAT
GCGGTCAAAGACATCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTG
GCTGGGAAGAAAGAATTCACTTGGATGGCCGAACGTTTTATATTGATCAT
AATAGCAAAATTACTCAGTGGGAAGACCCAAGACTGCAGAACCCAGCTAT
TACTGGTCCGGCTGTCCCTTACTCCAGAGAATTTAAGCAGAAATATGACT
ACTTCAGGAAGAAATTAAAGAAACCTGCTGATATCCCTAATAGGTTTGAA
ATGAAACTTCACAGAGATAACATATTTGAAGAGTCCTATCGGAGAATTAT
GTCCGTGAAAAGACCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTG
AATCAGAGAAAGGTCTTGACTATGGGGGTGTGGCCAGAGAATGGTTCTTC
TTACTGTCCAAAGAGATGTTCAACCCCTACTACGGCCTCTTTGAGTACTC
TGCCACGGACAACTACACCCTTCAGATCAACCCTAATTCAGGCCTCTGTA
ATGAGGATCATTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTG
GCCGTATTTCATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTA
CAAGATGATGTTGGGAAAGCAGATAACCCTGAATGACATGGAATCTGTGG
ATAGTGAATATTACAACTCTTTGAAATGGATCCTGGAGAATGACCCTACT
GAGCTGGACCTCATGTTCTGCATAGACGAAGAAAACTTTGGACAGACATA
TCAAGTGGATTTGAAGCCCAATGGGTCAGAAATAATGGTCACAAATGAAA
ACAAAAGGGAATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGG
GTCCAGAAGCAGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCC
TATTGATTTGATTAAAATTTTTGACGAAAATGAGCTGGAGTTGCTCATGT
GCGGCCTCGGTGATGTGGATGTGAATGACTGGAGACAGCATTCTATTTAC
AAGAACGGCTACTGCCCAAACCACCCCGTCATTCAGTGGTTCTGGAAGGC
TGTGCTACTCATGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTTGTCA
CAGGGACATCGCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCC
AATGGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACT
GCCCAGAGCTCACACATGCTTTAATCGCCTTGACTTACCTCCATATGAAA
CCTTTGAAGATTTACGAGAGAAACTTCTCATGGCCGTGGAAAATGCTCAA
GGATTTGAAGGGGTGGATTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAASVMDICR
IRPSAAERPYTFKDFLLRPRSHKSRVKGFLRLKMAHMPKNGGQDEENSDQ
RDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVNNLGRTYYVNHNN
RTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEG
GDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELCRRLQ
ITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSS
AVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRP
IMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPV
RRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFI
DHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFY
IDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPN
RFEMKLHRDNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVARE
WFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRV
AGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILEN
DPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRF
VNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQH
SIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAEL
YGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVE
NAQGFEGVD*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3917
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.