cDNA Clone NEDD4.2b1

• Plasmid: pEBG6P-Nedd4.2b1 S221A
• Parent Plasmid: pEBG6P
• Expressed: GST-Nedd4.2b1 S221A
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAG
AAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCCATA
TGCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGAC
ATGGAGCATGGATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCA
CCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAG
TGAACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACT
CAGTGGCACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAA
CATCAGACAGATCAACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCA
GGCACATCAGCGAAGACTTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTC
CCCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAATATCGCTGGAGACTC
TCTCGGTCTGGCTCTGCCCCCACCGCCGGCCTCCCCAGGATCTCGGACCA
GCCCTCAGGAGCTGTCAGAGGAACTATGCAGAAGGCTTCAGATCACTCCA
GACTCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTC
AAGGTTGAGGTCATGCGCTGTCACCGACGCAGTTGCAGAACAGGGCCATC
TACCACCGCCCAGTGCCCCAGCTGGGAGAGCGCGTTCATCAACTGTCACG
GGTGGTGAGGAACCAACGCCATCAGTGGCCTATGTACATACCACGCCGGG
TCTGCCCTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACATACT
ATGTCAATCATAACAATCGAACCACAACTTGGACTCGACCTATCATGCAG
CTTGCAGAAGATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCT
AATCGAGCCTCAGATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAA
CTTTATCTGCCCCGCTGGAGGGTGCCAAGGACTCACCCGTACGTCGGGCT
GTGAAAGACACCCTTTCCAACCCACAGTCCCCACAGCCATCACCTTACAA
CTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCTTGCCGCCCGGCT
GGGAAATGAGGATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAAC
ACAAAGACTACAACCTGGGAAGATCCACGTTTGAAGTTTCCAGTACATAT
GCGGTCAAAGACATCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTG
GCTGGGAAGAAAGAATTCACTTGGATGGCCGAACGTTTTATATTGATCAT
AATAGCAAAATTACTCAGTGGGAAGACCCAAGACTGCAGAACCCAGCTAT
TACTGGTCCGGCTGTCCCTTACTCCAGAGAATTTAAGCAGAAATATGACT
ACTTCAGGAAGAAATTAAAGAAACCTGCTGATATCCCTAATAGGTTTGAA
ATGAAACTTCACAGAGATAACATATTTGAAGAGTCCTATCGGAGAATTAT
GTCCGTGAAAAGACCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTG
AATCAGAGAAAGGTCTTGACTATGGGGGTGTGGCCAGAGAATGGTTCTTC
TTACTGTCCAAAGAGATGTTCAACCCCTACTACGGCCTCTTTGAGTACTC
TGCCACGGACAACTACACCCTTCAGATCAACCCTAATTCAGGCCTCTGTA
ATGAGGATCATTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTG
GCCGTATTTCATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTA
CAAGATGATGTTGGGAAAGCAGATAACCCTGAATGACATGGAATCTGTGG
ATAGTGAATATTACAACTCTTTGAAATGGATCCTGGAGAATGACCCTACT
GAGCTGGACCTCATGTTCTGCATAGACGAAGAAAACTTTGGACAGACATA
TCAAGTGGATTTGAAGCCCAATGGGTCAGAAATAATGGTCACAAATGAAA
ACAAAAGGGAATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGG
GTCCAGAAGCAGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCC
TATTGATTTGATTAAAATTTTTGACGAAAATGAGCTGGAGTTGCTCATGT
GCGGCCTCGGTGATGTGGATGTGAATGACTGGAGACAGCATTCTATTTAC
AAGAACGGCTACTGCCCAAACCACCCCGTCATTCAGTGGTTCTGGAAGGC
TGTGCTACTCATGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTTGTCA
CAGGGACATCGCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCC
AATGGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACT
GCCCAGAGCTCACACATGCTTTAATCGCCTTGACTTACCTCCATATGAAA
CCTTTGAAGATTTACGAGAGAAACTTCTCATGGCCGTGGAAAATGCTCAA
GGATTTGAAGGGGTGGATTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAERPY
TFKDFLLRPRSHKSRVKGFLRLKMAHMPKNGGQDEENSDQRDDMEHGWEV
VDSNDSASQHQEELPPPPLPPGWEEKVNNLGRTYYVNHNNRTTQWHRPSL
MDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETI
SEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELCRRLQITPDSNGEQF
SSLIQREPSSRLRSCAVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPTP
SVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAS
GSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSN
PQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWE
DPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQW
EDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRDN
IFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMF
NPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKL
LDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFC
IDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNA
FLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPN
HPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFT
IEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3910
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag.