cDNA Clone NEDD4.2b1

• Plasmid: pFBHTb nedd4.2b1 S221A S327A S328A
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-nedd4.2b1 S221A S327A S328A
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAG
AAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCCATA
TGCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGAC
ATGGAGCATGGATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCA
CCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAG
TGAACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACT
CAGTGGCACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAA
CATCAGACAGATCAACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCA
GGCACATCAGCGAAGACTTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTC
CCCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAATATCGCTGGAGACTC
TCTCGGTCTGGCTCTGCCCCCACCGCCGGCCTCCCCAGGATCTCGGACCA
GCCCTCAGGAGCTGTCAGAGGAACTATGCAGAAGGCTTCAGATCACTCCA
GACTCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTC
AAGGTTGAGGTCATGCGCTGTCACCGACGCAGTTGCAGAACAGGGCCATC
TACCACCGCCCAGTGCCCCAGCTGGGAGAGCGCGTTCATCAACTGTCACG
GGTGGTGAGGAACCAACGCCATCAGTGGCCTATGTACATACCACGCCGGG
TCTGCCCTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACATACT
ATGTCAATCATAACAATCGAACCACAACTTGGACTCGACCTATCATGCAG
CTTGCAGAAGATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCT
AATCGAGCCTCAGATCCGCCGGCCTCGTAGCCTCGCCGCGCCAACAGTAA
CTTTATCTGCCCCGCTGGAGGGTGCCAAGGACTCACCCGTACGTCGGGCT
GTGAAAGACACCCTTTCCAACCCACAGTCCCCACAGCCATCACCTTACAA
CTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCTTGCCGCCCGGCT
GGGAAATGAGGATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAAC
ACAAAGACTACAACCTGGGAAGATCCACGTTTGAAGTTTCCAGTACATAT
GCGGTCAAAGACATCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTG
GCTGGGAAGAAAGAATTCACTTGGATGGCCGAACGTTTTATATTGATCAT
AATAGCAAAATTACTCAGTGGGAAGACCCAAGACTGCAGAACCCAGCTAT
TACTGGTCCGGCTGTCCCTTACTCCAGAGAATTTAAGCAGAAATATGACT
ACTTCAGGAAGAAATTAAAGAAACCTGCTGATATCCCTAATAGGTTTGAA
ATGAAACTTCACAGAGATAACATATTTGAAGAGTCCTATCGGAGAATTAT
GTCCGTGAAAAGACCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTG
AATCAGAGAAAGGTCTTGACTATGGGGGTGTGGCCAGAGAATGGTTCTTC
TTACTGTCCAAAGAGATGTTCAACCCCTACTACGGCCTCTTTGAGTACTC
TGCCACGGACAACTACACCCTTCAGATCAACCCTAATTCAGGCCTCTGTA
ATGAGGATCATTTGTCGTACTTCACTTTTATTGGAAGAGTTGCTGGTCTG
GCCGTATTTCATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTA
CAAGATGATGTTGGGAAAGCAGATAACCCTGAATGACATGGAATCTGTGG
ATAGTGAATATTACAACTCTTTGAAATGGATCCTGGAGAATGACCCTACT
GAGCTGGACCTCATGTTCTGCATAGACGAAGAAAACTTTGGACAGACATA
TCAAGTGGATTTGAAGCCCAATGGGTCAGAAATAATGGTCACAAATGAAA
ACAAAAGGGAATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGG
GTCCAGAAGCAGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCC
TATTGATTTGATTAAAATTTTTGACGAAAATGAGCTGGAGTTGCTCATGT
GCGGCCTCGGTGATGTGGATGTGAATGACTGGAGACAGCATTCTATTTAC
AAGAACGGCTACTGCCCAAACCACCCCGTCATTCAGTGGTTCTGGAAGGC
TGTGCTACTCATGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTTGTCA
CAGGGACATCGCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCC
AATGGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACT
GCCCAGAGCTCACACATGCTTTAATCGCCTTGACTTACCTCCATATGAAA
CCTTTGAAGATTTACGAGAGAAACTTCTCATGGCCGTGGAAAATGCTCAA
GGATTTGAAGGGGTGGATTAAGCGGCCGC

Amino Acid Sequence

Protein Sequence of expressed construct
Precission Protease site is underlined, Thrombin is in italics MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAASVMDICR
IRPSAAERPYTFKDFLLRPRSHKSRVKGFLRLKMAHMPKNGGQDEENSDQ
RDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVNNLGRTYYVNHNN
RTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEG
GDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELCRRLQ
ITPDSNGEQFSSLIQREPSSRLRSCAVTDAVAEQGHLPPPSAPAGRARSS
TVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRP
IMQLAEDGASGSATNSNNHLIEPQIRRPRSLAAPTVTLSAPLEGAKDSPV
RRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFI
DHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFY
IDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPN
RFEMKLHRDNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVARE
WFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRV
AGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILEN
DPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRF
VNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQH
SIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAEL
YGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVE
NAQGFEGVD*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3898
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6his tag.