cDNA Clone SGK1

• Plasmid: pCMV5-HA SGK1 K127A S422D
• Parent Plasmid: pCMV5
• Expressed: HA-SGK1 K127A S422D
• Genbank: AAH01263
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCAGATCCGCCACCATGTATCCATATGATGTTCCAGATTATGCTAC
GGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGG
GCATGGTGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTCTG
AACGACTTTATTCAGAAGATTGCCAATAACTCCTATGCATGCAAACACCC
TGAAGTTCAGTCCATCTTGAAGATCTCCCAACCTCAGGAGCCTGAGCTTA
TGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCTCAGCAAATCAACCTT
GGCCCGTCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTCTTGAA
AGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGG
CAGAAGAAGTGTTCTATGCAGTCGCTGTTTTACAGAAGAAAGCAATCCTG
AAAAAGAAAGAGGAGAAGCATATTATGTCGGAGCGGAATGTTCTGTTGAA
GAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTG
CTGACAAATTGTACTTTGTCCTAGACTACATTAATGGTGGAGAGTTGTTC
TACCATCTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGTTTCTA
TGCTGCTGAAATAGCCAGTGCCTTGGGCTACCTGCATTCACTGAACATCG
TTTATAGAGACTTAAAACCAGAGAATATTTTGCTAGATTCACAGGGACAC
ATTGTCCTTACTGATTTCGGACTCTGCAAGGAGAACATTGAACACAACAG
CACAACATCCACCTTCTGTGGCACGCCGGAGTATCTCGCACCTGAGGTGC
TTCATAAGCAGCCTTATGACAGGACTGTGGACTGGTGGTGCCTGGGAGCT
GTCTTGTATGAGATGCTGTATGGCCTGCCGCCTTTTTATAGCCGAAACAC
AGCTGAAATGTACGACAACATTCTGAACAAGCCTCTCCAGCTGAAACCAA
ATATTACAAATTCCGCAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGAC
AGGACAAAGCGGCTCGGGGCCAAGGATGACTTCATGGAGATTAAGAGTCA
TGTCTTCTTCTCCTTAATTAACTGGGATGATCTCATTAATAAGAAGATTA
CTCCCCCTTTTAACCCAAATGTGAGTGGGCCCAACGACCTACGGCACTTT
GACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAGTCCCC
TGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCC
TAGGCTTTGACTATGCGCCTCCCACGGACTCTTTCCTCTGAGTCGAC

Amino Acid Sequence

MYPYDVPDYATVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQK
IANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNP
HAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVAVLQKKAILKKKEEK
HIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRE
RCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDF
GLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEML
YGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLG
AKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTE
EPVPNSIGKSPDSVLVTASVKEAAEAFLGFDYAPPTDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3894
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.