cDNA Clone Ubiquitin

• Plasmid: pCMV-STREP-HA Ubiquitin K48R K63R
• Parent Plasmid: pCMV5 23aa
• Expressed: STREP HA Ubiquitin K48R K63R
• Genbank: P62988
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGATCTT
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MASSLRQILDSQKMEWRSNAGGSEFRSGTTRIDKLACLQVDSRGSATMYP
YDVPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI
FAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG**

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3884
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal STREP/HA tag for mammalian expression. (Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7480-5)