cDNA Clone AMPK (mouse)

• Plasmid: pGEX-AMPK 3-307
• Parent Plasmid: pGEX4T1
• Expressed: GST-AMPK 3-307 T172D
• Genbank: AAC52355.1
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCGAATTCGCCATGGAGCAGAAGCTTATCTCCGAGGAGGACCTCGG
TGGCGGCGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGCCACTACA
TCCTGGGGGACACGCTGGGCGTCGGCACCTTCGGGAAAGTGAAGGTGGGC
AAGCACGAGTTGACTGGACATAAAGTTGCTGTGAAGATACTCAACCGGCA
GAAGATTCGAAGCCTGGACGTGGTCGGGAAAATCCGCAGAGAGATCCAGA
ACCTGAAGCTTTTCAGGCACCCTCATATAATCAAACTGTACCAGGTCATC
AGTACACCGTCTGATATTTTCATGGTCATGGAATATGTCTCAGGAGGAGA
GCTATTTGATTATATCTGTAAAAATGGAAGGTTGGACGAAAAGGAGAGTC
GACGTCTGTTCCAGCAGATCCTTTCTGGTGTGGACTATTGTCACAGGCAT
ATGGTGGTCCACAGAGATTTGAAACCTGAAAACGTCCTGCTTGATGCACA
CATGAATGCAAAGATAGCCGACTTCGGTCTTTCAAACATGATGTCAGATG
GTGAATTTTTAAGAACGAGCTGTGGCTCGCCCAATTATGCTGCACCAGAA
GTAATTTCAGGAAGATTGTACGCAGGCCCTGAAGTAGACATCTGGAGCAG
CGGGGTCATTCTCTATGCTTTGCTGTGTGGAACTCTCCCTTTTGATGATG
ACCACGTGCCAACTCTTTTTAAGAAGATATGTGACGGGATATTTTATACC
CCTCAGTATTTGAATCCCTCTGTAATAAGCCTTTTGAAGCATATGCTGCA
GGTAGATCCTATGAAGAGGGCCACAATAAAAGATATCAGGGAACATGAAT
GGTTTAAGCAGGACCTTCCAAAATATCTCTTTCCTGAAGACCCGTCTTAT
AGTTCAACCATGATTGATGATGAAGCCTTAAAAGAAGTGTGTGAGAAGTT
CGAGTGCTCAGAGGAGGAGGTCCTCAGATCCATCACACTGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSEFAMEQKLISEEDLGGGEKQKHDG
RVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVG
KIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNG
RLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFG
LSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLC
GTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATI
KDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLR
SITLAAARDRLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3872
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: General Description N terminal GST/C-Myc tag for bacterial expression. RSITLAAARDRLD added after AMPK sequence in expressed protein.