cDNA Clone PTEN

• Plasmid: pGEX6P-PTEN
• Parent Plasmid: pGEX6P1
• Expressed: GST-PTEN
• Genbank: CR450306
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAG
ATATCAAGAGGATGGATTCGACTTAGACTTGACCTATATTTATCCAAACA
TTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGCGTATACAGGAAC
AATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTA
CAAGATATACAATCTTTGTGCTGAAAGACATTATGACACCGCCAAATTTA
ATTGCAGAGTTGCACAATATCCTTTTGAAGACCATAACCCACCACAGCTA
GAACTTATCAAACCCTTTTGTGAAGATCTTGACCAATGGCTAAGTGAAGA
TGACAATCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGAACTG
GTGTAATGATATGTGCATATTTATTACATCGGGGCAAATTTTTAAAGGCA
CAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGACCAGAGACAAAAAGGG
AGTAACTATTCCCAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGT
TAAAGAATCATCTGGATTATAGACCAGTGGCACTGTTGTTTCACAAGATG
ATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACTTGCAATCCTCAGTT
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA
CACGACGGGAAGACAAGTTCATGTACTTTGAGTTCCCTCAGCCGTTACCT
GTGTGTGGTGATATCAAAGTAGAGTTCTTCCACAAACAGAACAAGATGCT
AAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAG
GACCAGAGGAAACCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAA
GAAATCGATAGCATTTGCAGTATAGAGCGTGCAGATAATGACAAGGAATA
TCTAGTACTTACTTTAACAAAAAATGATCTTGACAAAGCAAATAAAGACA
AAGCCAACCGATACTTTTCTCCAAATTTTAAGGTGAAGCTGTACTTCACA
AAAACAGTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGT
AACACCAGATGTTAGTGACAACGAACCTGATCATTATAGATATTCTGACA
CCACTGACTCTGATCCAGAGAATGAACCTTTTGATGAAGATCAGCATACA
CAAATTACAAAAGTCTGAGGGATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTAIIKEIVSRNKRRYQED
GFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYN
LCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHV
AAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIP
SQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQ
LKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDK
MFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLT
LTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDV
SDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3870
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Calc Mol Weight : 73942 Daltons