cDNA Clone S6K2

• Plasmid: pFBHTb P70S6K2 1-397 T388E
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-P70S6K2 1-397 T388E
• Genbank: NM_003952
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCGTGTTTGATTTGGATTTGGAGACGGAGGAAGGCAG
CGAGGGCGAGGGCGAGCCAGAGCTCAGCCCCGCGGACGCATGTCCCCTTG
CCGAGTTGAGGGCAGCTGGCCTAGAGCCTGTGGGACACTATGAAGAGGTG
GAGCTGACTGAGACCAGCGTGAACGTTGGCCCAGAGCGCATCGGGCCCCA
CTGCTTTGAGCTGCTGCGTGTGCTGGGCAAGGGGGGCTATGGCAAGGTGT
TCCAGGTGCGAAAGGTGCAAGGCACCAACTTGGGCAAAATATATGCCATG
AAAGTCCTAAGGAAGGCCAAAATTGTGCGCAATGCCAAGGACACAGCACA
CACACGGGCTGAGCGGAACATTCTAGAGTCAGTGAAGCACCCCTTTATTG
TGGAACTGGCCTATGCCTTCCAGACTGGTGGCAAACTCTACCTCATCCTT
GAGTGCCTCAGTGGTGGCGAGCTCTTCACGCATCTGGAGCGAGAGGGCAT
CTTCCTGGAAGATACGGCCTGCTTCTACCTGGCTGAGATCACGCTGGCCC
TGGGCCATCTCCACTCCCAGGGCATCATCTACCGGGACCTCAAGCCCGAG
AACATCATGCTCAGCAGCCAGGGCCACATCAAACTGACCGACTTTGGACT
CTGCAAGGAGTCTATCCATGAGGGCGCCGTCACTCACACCTTCTGCGGCA
CCATTGAGTACATGGCCCCTGAGATTCTGGTGCGCAGTGGCCACAACCGG
GCTGTGGACTGGTGGAGCCTGGGGGCCCTGATGTACGACATGCTCACTGG
ATCGCCGCCCTTTACCGCAGAGAACCGGAAGAAAACCATGGATAAGATCA
TCAGGGGCAAGCTGGCATTGCCCCCCTACCTCACCCCAGATGCCCGGGAC
CTTGTCAAAAAGTTTCTGAAACGGAATCCCAGCCAGCGGATTGGGGGTGG
CCCAGGGGATGCTGCTGATGTGCAGAGACATCCCTTTTTCCGGCACATGA
ATTGGGACGACCTTCTGGCCTGGCGTGTGGACCCCCCTTTCAGGCCCTGT
CTGCAGTCAGAGGAGGACGTGAGCCAGTTTGATACCCGCTTCACACGGCA
GACGCCGGTGGACAGTCCTGATGACACAGCCCTCAGCGAGAGTGCCAACC
AGGCCTTCCTGGGCTTCGAATACGTGGCGCCGTCTGTCCTGGACAGCTAA
GCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAAVFDLDLETEEGSEGEGEPE
LSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHCFELLRV
LGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDTAHTRAERNI
LESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTAC
FYLAEITLALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHE
GAVTHTFCGTIEYMAPEILVRSGHNRAVDWWSLGALMYDMLTGSPPFTAE
NRKKTMDKIIRGKLALPPYLTPDARDLVKKFLKRNPSQRIGGGPGDAADV
QRHPFFRHMNWDDLLAWRVDPPFRPCLQSEEDVSQFDTRFTRQTPVDSPD
DTALSESANQAFLGFEYVAPSVLDS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3856
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression.; Calc Mol Weight : 47574 Daltons