cDNA Clone NDRG1

• Plasmid: pEGFP-C1-FLAG NDRG1 T346A T356A T366A
• Parent Plasmid: pEGFPC1
• Expressed: GFP-FLAG NDRG1 T346A T356A T366A
• Genbank: XP_005243
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGTCTCGGGAGAT
GCAGGATGTAGACCTCGCTGAGGTGAAGCCTTTGGTGGAGAAAGGGGAGA
CCATCACCGGCCTCCTGCAAGAGTTTGATGTCCAGGAGCAGGACATCGAG
ACTTTACATGGCTCTGTTCACGTCACGCTGTGTGGGACTCCCAAGGGAAA
CCGGCCTGTCATCCTCACCTACCATGACATCGGCATGAACCACAAAACCT
GCTACAACCCCCTCTTCAACTACGAGGACATGCAGGAGATCACCCAGCAC
TTTGCCGTCTGCCACGTGGACGCCCCTGGCCAGCAGGACGGCGCAGCCTC
CTTCCCCGCAGGGTACATGTACCCCTCCATGGATCAGCTGGCTGAAATGC
TTCCTGGAGTCCTTCAACAGTTTGGGCTGAAAAGCATTATTGGCATGGGA
ACAGGAGCAGGCGCCTACATCCTAACTCGATTTGCTCTAAACAACCCTGA
GATGGTGGAGGGCCTTGTCCTTATCAACGTGAACCCTTGTGCGGAAGGCT
GGATGGACTGGGCCGCCTCCAAGATCTCAGGATGGACCCAAGCTCTGCCG
GACATGGTGGTGTCCCACCTTTTTGGGAAGGAAGAAATGCAGAGTAACGT
GGAAGTGGTCCACACCTACCGCCAGCACATTGTGAATGACATGAACCCCG
GCAACCTGCACCTGTTCATCAATGCCTACAACAGCCGGCGCGACCTGGAG
ATTGAGCGACCAATGCCGGGAACCCACACAGTCACCCTGCAGTGCCCTGC
TCTGTTGGTGGTTGGGGACAGCTCGCCTGCAGTGGATGCCGTGGTGGAGT
GCAACTCAAAATTGGACCCAACAAAGACCACTCTCCTCAAGATGGCGGAC
TGTGGCGGCCTCCCGCAGATCTCCCAGCCGGCCAAGCTCGCTGAGGCCTT
CAAGTACTTCGTGCAGGGCATGGGATACATGCCCTCGGCTAGCATGACCC
GCCTGATGCGGTCCCGCACAGCCTCTGGTTCCAGCGTCACTTCTCTGGAT
GGCACCCGCAGCCGCTCGCACGCCAGCGAGGGCACCCGAAGCCGCTCCCA
CGCCAGCGAGGGCACCCGCAGCCGCTCGCACGCCAGCGAGGGGGCCCACC
TGGACATCACCCCCAACTCGGGTGCTGCTGGGAACAGCGCCGGGCCCAAG
TCCATGGAGGTCTCCTGCTAGGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSATMDYKDDDDKSREMQDVDLAEVKPLVEKGETITGLLQE
FDVQEQDIETLHGSVHVTLCGTPKGNRPVILTYHDIGMNHKTCYNPLFNY
EDMQEITQHFAVCHVDAPGQQDGAASFPAGYMYPSMDQLAEMLPGVLQQF
GLKSIIGMGTGAGAYILTRFALNNPEMVEGLVLINVNPCAEGWMDWAASK
ISGWTQALPDMVVSHLFGKEEMQSNVEVVHTYRQHIVNDMNPGNLHLFIN
AYNSRRDLEIERPMPGTHTVTLQCPALLVVGDSSPAVDAVVECNSKLDPT
KTTLLKMADCGGLPQISQPAKLAEAFKYFVQGMGYMPSASMTRLMRSRTA
SGSSVTSLDGTRSRSHASEGTRSRSHASEGTRSRSHASEGAHLDITPNSG
AAGNSAGPKSMEVSC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3850
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/FLAG for mammalian expression.