cDNA Clone WDR4

• Plasmid: pEBG6P-HA WDR4 TV1
• Parent Plasmid: pEBG6P
• Expressed: GST-HA WDR4 TV1
• Genbank: NP_061139
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGCGGG
CTCTGTGGGACTGGCGTTGTGCGGGCAGACGTTGGTGGTGCGGGGCGGCA
GCCGATTCCTGGCCACCTCCATAGCAAGCAGTGATGATGACAGCCTCTTC
ATCTATGACTGCAGTGCTGCAGAAAAGAAGTCACAAGAAAATAAAGGGGA
GGACGCGCCCTTGGACCAGGGGAGCGGTGCGATTCTGGCGTCCACCTTCT
CCAAGTCTGGCAGCTATTTTGCTTTAACCGATGACAGTAAGCGTCTGATT
CTTTTCCGTACAAAACCATGGCAATGTCTGAGTGTCAGGACCGTGGCAAG
GAGGTGTACAGCCCTGACTTTCATAGCCTCGGAGGAGAAGGTCTTGGTGG
CCGACAAGTCTGGAGACGTCTACTCCTTTTCGGTGCTGGAGCCACACGGG
TGTGGCCGTCTAGAGCTGGGGCACCTGTCTATGCTGTTAGATGTGGCTGT
GAGTCCTGATGACCGCTTCATCCTCACTGCCGACCGGGACGAGAAGATCC
GAGTCAGCTGGGCCGCGGCGCCCCATAGCATCGAGTCCTTCTGCTTGGGG
CACACAGAGTTTGTGAGCCGTATCTCCGTGGTGCCAACTCAGCCCGGGCT
GCTTCTGTCCTCCTCTGGGGACGGCACCCTGAGGCTCTGGGAGTACAGGA
GCGGCCGCCAGCTGCACTGCTGTCACCTGGCCAGTCTGCAGGAGCTGGTG
GACCCCCAGGCCCCCCAGAAGTTTGCCGCGTCCAGGATTGCATTCTGGTG
CCAGGAGAACTGCGTGGCGCTCCTGTGCGACGGCACTCCTGTGGTCTACA
TCTTCCAGCTGGACGCCCGCAGACAGCAGTTGGTGTACAGGCAGCAGCTG
GCGTTCCAGCACCAAGTGTGGGACGTGGCTTTCGAGGAGACCCAGGGGCT
GTGGGTGCTCCAGGACTGCCAGGAAGCCCCCCTGGTGCTCTACAGGCCTG
TGGGCGACCAGTGGCAGTCTGTTCCTGAGAGCACCGTGTTAAAGAAAGTC
TCTGGTGTTCTTCGTGGGAACTGGGCCATGCTGGAAGGCTCTGCCGGCGC
AGACGCCAGCTTCAGCAGTCTCTACAAGGCCACGTTCGACAACGTGACCT
CCTACCTGAAGAAGAAAGAGGAGAGACTGCAGCAGCAGCTAGAGAAGAAG
CAGCGGCGCCGGAGTCCCCCGCCTGGGCCCGACGGGCATGCCAAGAAGAT
GAGACCGGGGGAGGCGACGCTAAGTTGCTGAGGATCC

Amino Acid Sequence

Protein sequence. M1 of WDR4 TV1 is M250 of expressed protein. HA tag is in italics. Precisiion site is undelined. Thrombin site is in bold MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSATMYPYDVPDYAM
AGSVGLALCGQTLVVRGGSRFLATSIASSDDDSLFIYDCSAAEKKSQENK
GEDAPLDQGSGAILASTFSKSGSYFALTDDSKRLILFRTKPWQCLSVRTV
ARRCTALTFIASEEKVLVADKSGDVYSFSVLEPHGCGRLELGHLSMLLDV
AVSPDDRFILTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRISVVPTQP
GLLLSSSGDGTLRLWEYRSGRQLHCCHLASLQELVDPQAPQKFAASRIAF
WCQENCVALLCDGTPVVYIFQLDARRQQLVYRQQLAFQHQVWDVAFEETQ
GLWVLQDCQEAPLVLYRPVGDQWQSVPESTVLKKVSGVLRGNWAMLEGSA
GADASFSSLYKATFDNVTSYLKKKEERLQQQLEKKQRRRSPPPGPDGHAK
KMRPGEATLSC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3845
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA for mammalian expression.; Calc Mol Weight : 74247 Daltons