cDNA Clone NIK

• Plasmid: pCMV-3HA NIK
• Parent Plasmid: pCMV5 3HA
• Expressed: 3HA NIK
• Genbank: Q99558
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGT
GGGGCAGCAGAAGGAACTCCCCAAGCCAAAGGAGAAGACGCCGCCACTGG
GGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCT
GTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGGCAC
AGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCATCTCTATCATCGCCC
AGGCTGAGTGTGAGAATAGCCAAGAGTTCAGCCCCACCTTTTCAGAACGC
ATTTTCATCGCTGGGTCCAAACAGTACAGCCAGTCCGAGAGTCTTGATCA
GATCCCCAACAATGTGGCCCATGCTACAGAGGGCAAAATGGCCCGTGTGT
GTTGGAAGGGAAAGCGTCGCAGCAAAGCCCGGAAGAAACGGAAGAAGAAG
AGCTCAAAGTCCCTGGCTCATGCAGGAGTGGCCTTGGCCAAACCCCTCCC
CAGGACCCCTGAGCAGGAGAGCTGCACCATCCCAGTGCAGGAGGATGAGT
CTCCACTCGGCGCCCCATATGTTAGAAACACCCCGCAGTTCACCAAGCCT
CTGAAGGAACCAGGCCTTGGGCAACTCTGTTTTAAGCAGCTTGGCGAGGG
CCTACGGCCGGCTCTGCCTCGATCAGAACTCCACAAACTGATCAGCCCCT
TGCAATGTCTGAACCACGTGTGGAAACTGCACCACCCCCAGGACGGAGGC
CCCCTGCCCCTGCCCACGCACCCCTTCCCCTATAGCAGACTGCCTCATCC
CTTCCCATTCCACCCTCTCCAGCCCTGGAAACCTCACCCTCTGGAGTCCT
TCCTGGGCAAACTGGCCTGTGTAGACAGCCAGAAACCCTTGCCTGACCCA
CACCTGAGCAAACTGGCCTGTGTAGACAGTCCAAAGCCCCTGCCTGGCCC
ACACCTGGAGCCCAGCTGCCTGTCTCGTGGTGCCCATGAGAAGTTTTCTG
TGGAGGAATACCTAGTGCATGCTCTGCAAGGCAGCGTGAGCTCAAGCCAG
GCCCACAGCCTGACCAGCCTGGCCAAGACCTGGGCAGCAAGGGGCTCCAG
ATCCCGGGAGCCCAGCCCCAAAACTGAGGACAACGAGGGTGTCCTGCTCA
CTGAGAAACTCAAGCCAGTGGATTATGAGTACCGAGAAGAAGTCCACTGG
GCCACGCACCAGCTCCGCCTGGGCAGAGGCTCCTTCGGAGAGGTGCACAG
GATGGAGGACAAGCAGACTGGCTTCCAGTGCGCTGTCAAAAAGGTGCGGC
TGGAAGTATTTCGGGCAGAGGAGCTGATGGCATGTGCAGGATTGACCTCA
CCCAGAATTGTCCCTTTGTATGGAGCTGTGAGAGAAGGGCCTTGGGTCAA
CATCTTCATGGAGCTGCTGGAAGGTGGCTCCCTGGGCCAGCTGGTCAAGG
AGCAGGGCTGTCTCCCAGAGGACCGGGCCCTGTACTACCTGGGCCAGGCC
CTGGAGGGTCTGGAATACCTCCACTCACGAAGGATTCTGCATGGGGACGT
CAAAGCTGACAACGTGCTCCTGTCCAGCGATGGGAGCCACGCAGCCCTCT
GTGACTTTGGCCATGCTGTGTGTCTTCAACCTGATGGCCTGGGAAAGTCC
TTGCTCACAGGGGACTACATCCCTGGCACAGAGACCCACATGGCTCCGGA
GGTGGTGCTGGGCAGGAGCTGCGACGCCAAGGTGGATGTCTGGAGCAGCT
GCTGTATGATGCTGCACATGCTCAACGGCTGCCACCCCTGGACTCAGTTC
TTCCGAGGGCCGCTCTGCCTCAAGATTGCCAGCGAGCCTCCGCCTGTGAG
GGAGATCCCACCCTCCTGCGCCCCTCTCACAGCCCAGGCCATCCAAGAGG
GGCTGAGGAAAGAGCCCATCCACCGCGTGTCTGCAGCGGAGCTGGGAGGG
AAGGTGAACCGGGCACTACAGCAAGTGGGAGGTCTGAAGAGCCCTTGGAG
GGGAGAATATAAAGAACCAAGACATCCACCGCCAAATCAAGCCAATTACC
ACCAGACCCTCCATGCCCAGCCGAGAGAGCTTTCGCCAAGGGCCCCAGGG
CCCCGGCCAGCTGAGGAGACAACAGGCAGAGCCCCTAAGCTCCAGCCTCC
TCTCCCACCAGAGCCCCCAGAGCCAAACAAGTCTCCTCCCTTGACTTTGA
GCAAGGAGGAGTCTGGGATGTGGGAACCCTTACCTCTGTCCTCCCTGGAG
CCAGCCCCTGCCAGAAACCCCAGCTCACCAGAGCGGAAAGCAACCGTCCC
GGAGCAGGAACTGCAGCAGCTGGAAATAGAATTATTCCTCAACAGCCTGT
CCCAGCCATTTTCTCTGGAGGAGCAGGAGCAAATTCTCTCGTGCCTCAGC
ATCGACAGCCTCTCCCTGTCGGATGACAGTGAGAAGAACCCATCAAAGGC
CTCTCAAAGCTCGCGGGACACCCTGAGCTCAGGCGTACACTCCTGGAGCA
GCCAGGCCGAGGCTCGAAGCTCCAGCTGGAACATGGTGCTGGCCCGGGGG
CGGCCCACCGACACCCCAAGCTATTTCAATGGTGTGAAAGTCCAAATACA
GTCTCTTAATGGTGAACACCTGCACATCCGGGAGTTCCACCGGGTCAAAG
TGGGAGACATCGCCACTGGCATCAGCAGCCAGATCCCAGCTGCAGCCTTC
AGCTTGGTGACCAAAGACGGGCAGCCTGTTCGCTACGACATGGAGGTGCC
AGACTCGGGCATCGACCTGCAGTGCACACTGGCCCCTGATGGCAGCTTCG
CCTGGAGCTGGAGGGTCAAGCATGGCCAGCTGGAGAACAGGCCCTAAGAA
TTC

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFRSGTTRIDKLACLQVDSR
GSAVMEMACPGAPGSAVGQQKELPKPKEKTPPLGKKQSSVYKLEAVEKSP
VFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSER
IFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKK
SSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKP
LKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGG
PLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDP
HLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSSQ
AHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHW
ATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTS
PRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQA
LEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKS
LLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQF
FRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGG
KVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPG
PRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLE
PAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLS
IDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARG
RPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAF
SLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3833
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 3HA for mammalian expression.; Calc Mol Weight : 109964 Daltons