cDNA Clone NIK

• Plasmid: pFBHTb NIK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-NIK
• Genbank: Q99558
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGT
GGGGCAGCAGAAGGAACTCCCCAAGCCAAAGGAGAAGACGCCGCCACTGG
GGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCT
GTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGGCAC
AGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCATCTCTATCATCGCCC
AGGCTGAGTGTGAGAATAGCCAAGAGTTCAGCCCCACCTTTTCAGAACGC
ATTTTCATCGCTGGGTCCAAACAGTACAGCCAGTCCGAGAGTCTTGATCA
GATCCCCAACAATGTGGCCCATGCTACAGAGGGCAAAATGGCCCGTGTGT
GTTGGAAGGGAAAGCGTCGCAGCAAAGCCCGGAAGAAACGGAAGAAGAAG
AGCTCAAAGTCCCTGGCTCATGCAGGAGTGGCCTTGGCCAAACCCCTCCC
CAGGACCCCTGAGCAGGAGAGCTGCACCATCCCAGTGCAGGAGGATGAGT
CTCCACTCGGCGCCCCATATGTTAGAAACACCCCGCAGTTCACCAAGCCT
CTGAAGGAACCAGGCCTTGGGCAACTCTGTTTTAAGCAGCTTGGCGAGGG
CCTACGGCCGGCTCTGCCTCGATCAGAACTCCACAAACTGATCAGCCCCT
TGCAATGTCTGAACCACGTGTGGAAACTGCACCACCCCCAGGACGGAGGC
CCCCTGCCCCTGCCCACGCACCCCTTCCCCTATAGCAGACTGCCTCATCC
CTTCCCATTCCACCCTCTCCAGCCCTGGAAACCTCACCCTCTGGAGTCCT
TCCTGGGCAAACTGGCCTGTGTAGACAGCCAGAAACCCTTGCCTGACCCA
CACCTGAGCAAACTGGCCTGTGTAGACAGTCCAAAGCCCCTGCCTGGCCC
ACACCTGGAGCCCAGCTGCCTGTCTCGTGGTGCCCATGAGAAGTTTTCTG
TGGAGGAATACCTAGTGCATGCTCTGCAAGGCAGCGTGAGCTCAAGCCAG
GCCCACAGCCTGACCAGCCTGGCCAAGACCTGGGCAGCAAGGGGCTCCAG
ATCCCGGGAGCCCAGCCCCAAAACTGAGGACAACGAGGGTGTCCTGCTCA
CTGAGAAACTCAAGCCAGTGGATTATGAGTACCGAGAAGAAGTCCACTGG
GCCACGCACCAGCTCCGCCTGGGCAGAGGCTCCTTCGGAGAGGTGCACAG
GATGGAGGACAAGCAGACTGGCTTCCAGTGCGCTGTCAAAAAGGTGCGGC
TGGAAGTATTTCGGGCAGAGGAGCTGATGGCATGTGCAGGATTGACCTCA
CCCAGAATTGTCCCTTTGTATGGAGCTGTGAGAGAAGGGCCTTGGGTCAA
CATCTTCATGGAGCTGCTGGAAGGTGGCTCCCTGGGCCAGCTGGTCAAGG
AGCAGGGCTGTCTCCCAGAGGACCGGGCCCTGTACTACCTGGGCCAGGCC
CTGGAGGGTCTGGAATACCTCCACTCACGAAGGATTCTGCATGGGGACGT
CAAAGCTGACAACGTGCTCCTGTCCAGCGATGGGAGCCACGCAGCCCTCT
GTGACTTTGGCCATGCTGTGTGTCTTCAACCTGATGGCCTGGGAAAGTCC
TTGCTCACAGGGGACTACATCCCTGGCACAGAGACCCACATGGCTCCGGA
GGTGGTGCTGGGCAGGAGCTGCGACGCCAAGGTGGATGTCTGGAGCAGCT
GCTGTATGATGCTGCACATGCTCAACGGCTGCCACCCCTGGACTCAGTTC
TTCCGAGGGCCGCTCTGCCTCAAGATTGCCAGCGAGCCTCCGCCTGTGAG
GGAGATCCCACCCTCCTGCGCCCCTCTCACAGCCCAGGCCATCCAAGAGG
GGCTGAGGAAAGAGCCCATCCACCGCGTGTCTGCAGCGGAGCTGGGAGGG
AAGGTGAACCGGGCACTACAGCAAGTGGGAGGTCTGAAGAGCCCTTGGAG
GGGAGAATATAAAGAACCAAGACATCCACCGCCAAATCAAGCCAATTACC
ACCAGACCCTCCATGCCCAGCCGAGAGAGCTTTCGCCAAGGGCCCCAGGG
CCCCGGCCAGCTGAGGAGACAACAGGCAGAGCCCCTAAGCTCCAGCCTCC
TCTCCCACCAGAGCCCCCAGAGCCAAACAAGTCTCCTCCCTTGACTTTGA
GCAAGGAGGAGTCTGGGATGTGGGAACCCTTACCTCTGTCCTCCCTGGAG
CCAGCCCCTGCCAGAAACCCCAGCTCACCAGAGCGGAAAGCAACCGTCCC
GGAGCAGGAACTGCAGCAGCTGGAAATAGAATTATTCCTCAACAGCCTGT
CCCAGCCATTTTCTCTGGAGGAGCAGGAGCAAATTCTCTCGTGCCTCAGC
ATCGACAGCCTCTCCCTGTCGGATGACAGTGAGAAGAACCCATCAAAGGC
CTCTCAAAGCTCGCGGGACACCCTGAGCTCAGGCGTACACTCCTGGAGCA
GCCAGGCCGAGGCTCGAAGCTCCAGCTGGAACATGGTGCTGGCCCGGGGG
CGGCCCACCGACACCCCAAGCTATTTCAATGGTGTGAAAGTCCAAATACA
GTCTCTTAATGGTGAACACCTGCACATCCGGGAGTTCCACCGGGTCAAAG
TGGGAGACATCGCCACTGGCATCAGCAGCCAGATCCCAGCTGCAGCCTTC
AGCTTGGTGACCAAAGACGGGCAGCCTGTTCGCTACGACATGGAGGTGCC
AGACTCGGGCATCGACCTGCAGTGCACACTGGCCCCTGATGGCAGCTTCG
CCTGGAGCTGGAGGGTCAAGCATGGCCAGCTGGAGAACAGGCCCTAAGAA
TTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSAVMEMACPGAPGSAVGQQKELP
KPKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEA
GPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAH
ATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQES
CTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPR
SELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQ
PWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCL
SRGAHEKFSVEEYLVHALQGSVSSSQAHSLTSLAKTWAARGSRSREPSPK
TEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTG
FQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLE
GGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLL
SSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSC
DAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCA
PLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPR
HPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPE
PNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQL
EIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDT
LSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHL
HIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQ
CTLAPDGSFAWSWRVKHGQLENRP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3832
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression expression.; Calc Mol Weight : 107270 Daltons