cDNA Clone NIK

• Plasmid: pGEX6P-1-NIK
• Parent Plasmid: pGEX6P1
• Expressed: GST-NIK
• Genbank: Q99558
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGT
GGGGCAGCAGAAGGAACTCCCCAAGCCAAAGGAGAAGACGCCGCCACTGG
GGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCT
GTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGGCAC
AGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCATCTCTATCATCGCCC
AGGCTGAGTGTGAGAATAGCCAAGAGTTCAGCCCCACCTTTTCAGAACGC
ATTTTCATCGCTGGGTCCAAACAGTACAGCCAGTCCGAGAGTCTTGATCA
GATCCCCAACAATGTGGCCCATGCTACAGAGGGCAAAATGGCCCGTGTGT
GTTGGAAGGGAAAGCGTCGCAGCAAAGCCCGGAAGAAACGGAAGAAGAAG
AGCTCAAAGTCCCTGGCTCATGCAGGAGTGGCCTTGGCCAAACCCCTCCC
CAGGACCCCTGAGCAGGAGAGCTGCACCATCCCAGTGCAGGAGGATGAGT
CTCCACTCGGCGCCCCATATGTTAGAAACACCCCGCAGTTCACCAAGCCT
CTGAAGGAACCAGGCCTTGGGCAACTCTGTTTTAAGCAGCTTGGCGAGGG
CCTACGGCCGGCTCTGCCTCGATCAGAACTCCACAAACTGATCAGCCCCT
TGCAATGTCTGAACCACGTGTGGAAACTGCACCACCCCCAGGACGGAGGC
CCCCTGCCCCTGCCCACGCACCCCTTCCCCTATAGCAGACTGCCTCATCC
CTTCCCATTCCACCCTCTCCAGCCCTGGAAACCTCACCCTCTGGAGTCCT
TCCTGGGCAAACTGGCCTGTGTAGACAGCCAGAAACCCTTGCCTGACCCA
CACCTGAGCAAACTGGCCTGTGTAGACAGTCCAAAGCCCCTGCCTGGCCC
ACACCTGGAGCCCAGCTGCCTGTCTCGTGGTGCCCATGAGAAGTTTTCTG
TGGAGGAATACCTAGTGCATGCTCTGCAAGGCAGCGTGAGCTCAAGCCAG
GCCCACAGCCTGACCAGCCTGGCCAAGACCTGGGCAGCAAGGGGCTCCAG
ATCCCGGGAGCCCAGCCCCAAAACTGAGGACAACGAGGGTGTCCTGCTCA
CTGAGAAACTCAAGCCAGTGGATTATGAGTACCGAGAAGAAGTCCACTGG
GCCACGCACCAGCTCCGCCTGGGCAGAGGCTCCTTCGGAGAGGTGCACAG
GATGGAGGACAAGCAGACTGGCTTCCAGTGCGCTGTCAAAAAGGTGCGGC
TGGAAGTATTTCGGGCAGAGGAGCTGATGGCATGTGCAGGATTGACCTCA
CCCAGAATTGTCCCTTTGTATGGAGCTGTGAGAGAAGGGCCTTGGGTCAA
CATCTTCATGGAGCTGCTGGAAGGTGGCTCCCTGGGCCAGCTGGTCAAGG
AGCAGGGCTGTCTCCCAGAGGACCGGGCCCTGTACTACCTGGGCCAGGCC
CTGGAGGGTCTGGAATACCTCCACTCACGAAGGATTCTGCATGGGGACGT
CAAAGCTGACAACGTGCTCCTGTCCAGCGATGGGAGCCACGCAGCCCTCT
GTGACTTTGGCCATGCTGTGTGTCTTCAACCTGATGGCCTGGGAAAGTCC
TTGCTCACAGGGGACTACATCCCTGGCACAGAGACCCACATGGCTCCGGA
GGTGGTGCTGGGCAGGAGCTGCGACGCCAAGGTGGATGTCTGGAGCAGCT
GCTGTATGATGCTGCACATGCTCAACGGCTGCCACCCCTGGACTCAGTTC
TTCCGAGGGCCGCTCTGCCTCAAGATTGCCAGCGAGCCTCCGCCTGTGAG
GGAGATCCCACCCTCCTGCGCCCCTCTCACAGCCCAGGCCATCCAAGAGG
GGCTGAGGAAAGAGCCCATCCACCGCGTGTCTGCAGCGGAGCTGGGAGGG
AAGGTGAACCGGGCACTACAGCAAGTGGGAGGTCTGAAGAGCCCTTGGAG
GGGAGAATATAAAGAACCAAGACATCCACCGCCAAATCAAGCCAATTACC
ACCAGACCCTCCATGCCCAGCCGAGAGAGCTTTCGCCAAGGGCCCCAGGG
CCCCGGCCAGCTGAGGAGACAACAGGCAGAGCCCCTAAGCTCCAGCCTCC
TCTCCCACCAGAGCCCCCAGAGCCAAACAAGTCTCCTCCCTTGACTTTGA
GCAAGGAGGAGTCTGGGATGTGGGAACCCTTACCTCTGTCCTCCCTGGAG
CCAGCCCCTGCCAGAAACCCCAGCTCACCAGAGCGGAAAGCAACCGTCCC
GGAGCAGGAACTGCAGCAGCTGGAAATAGAATTATTCCTCAACAGCCTGT
CCCAGCCATTTTCTCTGGAGGAGCAGGAGCAAATTCTCTCGTGCCTCAGC
ATCGACAGCCTCTCCCTGTCGGATGACAGTGAGAAGAACCCATCAAAGGC
CTCTCAAAGCTCGCGGGACACCCTGAGCTCAGGCGTACACTCCTGGAGCA
GCCAGGCCGAGGCTCGAAGCTCCAGCTGGAACATGGTGCTGGCCCGGGGG
CGGCCCACCGACACCCCAAGCTATTTCAATGGTGTGAAAGTCCAAATACA
GTCTCTTAATGGTGAACACCTGCACATCCGGGAGTTCCACCGGGTCAAAG
TGGGAGACATCGCCACTGGCATCAGCAGCCAGATCCCAGCTGCAGCCTTC
AGCTTGGTGACCAAAGACGGGCAGCCTGTTCGCTACGACATGGAGGTGCC
AGACTCGGGCATCGACCTGCAGTGCACACTGGCCCCTGATGGCAGCTTCG
CCTGGAGCTGGAGGGTCAAGCATGGCCAGCTGGAGAACAGGCCCTAAGAA
TTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAVMEMACPGAPGSAVGQQK
ELPKPKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEG
SEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNN
VAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPE
QESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPA
LPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFH
PLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEP
SCLSRGAHEKFSVEEYLVHALQGSVSSSQAHSLTSLAKTWAARGSRSREP
SPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDK
QTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFME
LLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADN
VLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLG
RSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPP
SCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYK
EPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPE
PPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQEL
QQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSS
RDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNG
EHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGI
DLQCTLAPDGSFAWSWRVKHGQLENRP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3831
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Calc Mol Weight : 130707 Daltons