cDNA Clone TAK1-S

• Plasmid: pEBG6P-TAK1 1-402
• Parent Plasmid: pEBG6P
• Expressed: GST-TAK1 1-402
• Genbank: NP_003179
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCGTCGTCTTCGGC
CGGTGAGATGATCGAAGCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCG
ACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGAAGAGGAGCCTTTGGA
GTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCTATTAAACAAAT
AGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAGTTAT
CCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAAT
CCAGTGTGTCTTGTGATGGAATATGCTGAAGGGGGCTCTTTATATAACGT
GCTGCATGGTGCTGAACCATTGCCATATTATACTGCTGCCCACGCAATGA
GTTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTATCTTCACAGCATGCAA
CCCAAAGCGCTAATTCACAGGGACCTGAAACCACCAAACTTACTGCTGGT
TGCAGGGGGGACGGTTCTAAAAATTTGTGATTTTGGTACAGCCTGTGACA
TTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCT
GAAGTTTTTGAAGGTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTG
GGGTATTATTCTTTGGGAAGTGATAACGCGTCGGAAACCCTTTGATGAGA
TTGGTGGCCCAGCTTTCCGAATCATGTGGGCTGTTCATAATGGTACTCGA
CCACCACTGATAAAAAATTTACCTAAGCCCATTGAGAGCCTGATGACTCG
TTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAGGAAATTGTGA
AAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTA
CAGTATCCTTGTCAGTATTCAGATGAAGGACAGAGCAACTCTGCCACCAG
TACAGGCTCATTCATGGACATTGCTTCTACAAATACGAGTAACAAAAGTG
ACACTAATATGGAGCAAGTTCCTGCCACAAATGATACTATTAAGCGCTTA
GAGTCAAAATTGTTGAAAAATCAGGCAAAGCAACAGAGTGAATCTGGACG
TTTAAGCTTGGGAGCCTCCCGTGGGAGCAGTGTGGAGAGCTTGCCCCCAA
CCTCTGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGCTAGG
ATCGCCGCAACCTGAGGCAACGGACAGCCAAGACGTAGATCCATCCAAGA
CTTGACTGTAACTGGAACAGAACCTGGTCAGGTGAGCAGTAGGTCATCCA
GTCCCAGTGTCAGAATGATTACTACCTCAGGACCAACCTCAGAAAAGCCA
ACTCGAAGTCATCCATGGACCCCTGATGATTCCACAGATACCAATGGATC
AGATAACTCCATCCCAATGGCTTATCTTACACTGGATCACCAACTACAGC
CTCTAGCACCGTGCCCAAACTCCAAAGAATCTATGGCAGTGTTTGAACAG
CATTGTAAAATGGCACAAGAATATATGAAAGTTCAAACAGAAATTGCATT
GTTATTACAGAGAAAGCAAGAACTAGTTGCAGAACTGGACCAGGATGAAA
AGGACCAGCAAAATACATCTCGCCTGGTACAGGAACATAAAAAGCTTTTA
GATGAAAACAAAAGCCTTTCTACTTACTACCAGCAATGCAAAAAACAACT
AGAGGTCATCAGAAGTCAGCAGCAGAAACGACAAGGCACTTCATGAGGAT
CC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSTASAASSSSSS
SAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIK
QIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLY
NVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLL
LVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVF
SWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLM
TRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSA
TSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSES
GRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIAAT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3824
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Calc Mol Weight : 71631 Daltons