cDNA Clone NEDD4-2

• Plasmid: pEBG6P-NEDD4-2
• Parent Plasmid: pEBG6P
• Expressed: GST-NEDD4-2
• Genbank: AY112985
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAG
AAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCCATA
TGCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGAC
ATGGAGCATGGATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCA
CCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAG
TGAACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACT
CAGTGGCACAGACCGAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAA
CATCAGACAGATCAACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCA
GGCACATCAGCGAAGACTTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTC
CCCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAATATCGCTGGAGACCC
TCTCGGTCTGGCTCTGCCCCCACCACCGGCCTCCCCAGGATCTCGGACCA
GCCCTCAGGAGCTGTCAGAGGAACTATGCAGAAGGCTTCAGATCACTCCA
GACTCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTC
AAGGTTGAGGTCATGCAGTGTCACCGACGCAGTTGCAGAACAGGGCCATC
TACCACCGCCATCAGTGGCCTATGTACATACCACGCCGGGTCTGCCCTCA
GGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACATACTATGTCAATCA
TAACAATCGAACCACAACTTGGACTCGACCTATCATGCAGCTTGCAGAAG
ATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCTAATCGAGCCT
CAGATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAACTTTATCTGC
CCCGCTGGAGGGTGCCAAGGACTCACCCGTACGTCGGGCTGTGAAAGACA
CCCTTTCCAACCCACAGTCCCCACAGCCATCACCTTACAACTCCCCCAAA
CCACAACACAAAGTCACACAGAGCTTCTTGCCGCCCGGCTGGGAAATGAG
GATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAACACAAAGACTA
CAACCTGGGAAGATCCACGTTTGAAGTTTCCAGTACATATGCGGTCAAAG
ACATCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTGGCTGGGAAGA
AAGAATTCACTTGGATGGCCGAACGTTTTATATTGATCATAATAGCAAAA
TTACTCAGTGGGAAGACCCAAGACTGCAGAACCCAGCTATTACTGGTCCG
GCTGTCCCTTACTCCAGAGAATTTAAGCAGAAATATGACTACTTCAGGAA
GAAATTAAAGAAACCTGCTGATATCCCCAATAGGTTTGAAATGAAACTTC
ACAGAGATAACATATTTGAAGAGTCCTATCGGAGAATTATGTCCGTGAAA
AGACCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTGAATCAGAGAA
AGGTCTTGACTATGGGGGTGTGGCCAGAGAATGGTTCTTCTTACTGTCCA
AAGAGATGTTCAACCCCTACTACGGCCTCTTTGAGTACTCTGCCACGGAC
AACTACACCCTTCAGATCAACCCTAATTCAGGCCTCTGTAATGAGGATCA
TTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTGGCCGTATTTC
ATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTACAAGATGATG
TTGGGAAAGCAGATAACCCTGAACGACATGGAATCTGTGGATAGTGAATA
TTACAACTCTTTGAAATGGATCCTGGAGAATGACCCTACTGAGCTGGACC
TCATGTTCTGCATAGACGAAGAAAACTTTGGACAGACATATCAAGTGGAT
TTGAAGCCCAATGGGTCAGAAATAATGGTCACAAATGAAAACAAAAGGGA
ATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGGGTCCAGAAGC
AGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCCTATTGATTTG
ATTAAAATTTTTGACGAAAATGAGCTGGAGTTGCTCATGTGCGGCCTCGG
TGATGTGGATGTGAATGACTGGAGACAGCATTCTATTTACAAGAACGGCT
ACTGCCCAAACCACCCCGTCATTCAGTGGTTCTGGAAGGCTGTGCTACTC
ATGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTTGTCACAGGGACATC
GCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCCAATGGTCCTC
AGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACTGCCCAGAGCT
CACACATGCTTTAATCGCCTTGACTTACCTCCATATGAAACCTTTGAAGA
TTTACGAGAGAAACTTCTCATGGCCGTGGAAAATGCTCAAGGATTTGAAG
GGGTGGATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAERPY
TFKDFLLRPRSHKSRVKGFLRLKMAHMPKNGGQDEENSDQRDDMEHGWEV
VDSNDSASQHQEELPPPPLPPGWEEKVNNLGRTYYVNHNNRTTQWHRPSL
MDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETI
SEEVNIAGDPLGLALPPPPASPGSRTSPQELSEELCRRLQITPDSNGEQF
SSLIQREPSSRLRSCSVTDAVAEQGHLPPPSVAYVHTTPGLPSGWEERKD
AKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRS
LSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQ
SFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPND
LGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSRE
FKQKYDYFRKKLKKPADIPNRFEMKLHRDNIFEESYRRIMSVKRPDVLKA
RLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQIN
PNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITL
NDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSE
IMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDEN
ELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRI
RLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRL
DLPPYETFEDLREKLLMAVENAQGFEGVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3805
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag. As genbank except starts at aa115.Y144H, D196N S278P S304C N597D – all in original EST.