cDNA Clone DAZAP1-b

• Plasmid: pGEX6P-1-6His DAZAP1b T269D T315D
• Parent Plasmid: pGEX6P1
• Expressed: GST-6His DAZAP1b T269D T315D
• Genbank: NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCATCATCACCATCACCATATGAACAACTCGGGCGCCGACGAGAT
CGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGCACGACCCAAGAGACTC
TGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTTATCATG
AAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAA
AGACCCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAG
ATGGCCGAAACATCGACCCCAAGCCATGCACACCCCGGGGGATGCAGCCG
GAGAGAACACGGCCGAAGGAAGGATGGCAGAAAGGACCCAGGAGCGATAA
CAGTAAATCAAATAAGATATTTGTCGGTGGAATTCCTCACAATTGTGGTG
AGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACGGAGGTA
GTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTAT
TACTTTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTC
ACGACATCATGGGCAAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGAC
AGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCG
GGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGT
GGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCG
ATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACC
CCCACCGTTCACCTCCTACATCGTGTCCGACCCTCCTGGAGGCTTTCCCC
CTCCCCAGGGCTTCCCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTT
GGCTACGGGCCTCCACCTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGT
TCCTCCTCCACCAGCCGATCCCGGGGCAGCACCTCTGGCTTTCCCACCGC
CTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCA
GACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTT
CGGACAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTC
CCTCCGTGCCAGGGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGA
CGAGGGCAGAACCACAACGTGCAAGGGTTCCACCCCTACCGACGCTAGGG
ATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHHHHHHMNNSGADEIGKLF
VGGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNC
VGTVLASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSN
KIFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFED
EQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPN
AANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFT
SYIVSDPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPP
ADPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGF
SDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3801
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/6His tag for bacterial expression.