cDNA Clone PP2A

• Synonyms: -
• Plasmid: pACYCDuet PP2A 6His PTPA
• Parent Plasmid: pACYC Duet1
• Expressed: PP2A 6His PTPA
• Genbank: NP_002706 and AAH11605
• Tag: 6His
• Species: Human

Sequence of Insert

DNA sequence of PTPA insert :
GGATCCGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGG
CCCCTCCAGCCACTCAGAACTTCATCATTCCAAAAAAGGAGATCCACACA
GTTCCAGACATGGGCAAATGGAAGCGTTCTCAGGCATACGCTGACTACAT
CGGATTCATCCTTACCCTCAACGAAGGTGTGAAGGGGAAGAAGCTGACCT
TCGAGTACAGAGTCTCCGAGGCCATTGAGAAACTAGTCGCTCTTCTCAAC
ACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCG
GTTTGGGAATAAGGCATACAGGACCTGGTATGCCAAACTTGATGAGGAAG
CAGAAAACTTGGTGGCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTG
CCTGAGGTGGCTGTTTACCTAAAGGAGTCAGTGGGGAACTCCACGCGCAT
TGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGTC
TCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATTGTCTTC
AAGGTGTTCAATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATA
CAGGATGGAGCCAGCCGGCAGCCAGGGAGTGTGGGGTCTGGATGACTTCC
AGTTTCTGCCCTTCATCTGGGGCAGTTCGCAGCTGATAGACCACCCATAC
CTGGAGCCCAGACACTTTGTGGATGAGAAGGCCGTGAATGAGAACCACAA
GGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACTG
GCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCCGTCCCT
TCCTGGTCCAAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTG
CCTGGAGAAGTTCCCTGTGATCCAGCACTTCAAGTTCGGGAGCCTGCTGC
CCATCCATCCTGTCACGTCGGGCTAGGAATTC

DNA sequence of PP2Ainsert:
CATATGGACGAGAAGGTGTTCACCAAGGAGCTGGACCAGTGGATCGAGCA
GCTGAACGAGTGCAAGCAGCTGTCCGAGTCCCAGGTCAAGAGCCTCTGCG
AGAAGGCTAAAGAAATCCTGACAAAAGAATCCAACGTGCAAGAGGTTCGA
TGTCCAGTTACTGTCTGTGGAGATGTGCATGGGCAATTTCATGATCTCAT
GGAACTGTTTAGAATTGGTGGCAAATCACCAGATACAAATTACTTGTTTA
TGGGAGATTATGTTGACAGAGGATATTATTCAGTTGAAACAGTTACACTG
CTTGTAGCTCTTAAGGTTCGTTACCGTGAACGCATCACCATTCTTCGAGG
GAATCATGAGAGCAGACAGATCACACAAGTTTATGGTTTCTATGATGAAT
GTTTAAGAAAATATGGAAATGCAAATGTTTGGAAATATTTTACAGATCTT
TTTGACTATCTTCCTCTCACTGCCTTGGTGGATGGGCAGATCTTCTGTCT
ACATGGTGGTCTCTCGCCATCTATAGATACACTGGATCATATCAGAGCAC
TTGATCGCCTACAAGAAGTTCCCCATGAGGGTCCAATGTGTGACTTGCTG
TGGTCAGATCCAGATGACCGTGGTGGTTGGGGTATATCTCCTCGAGGAGC
TGGTTACACCTTTGGGCAAGATATTTCTGAGACATTTAATCATGCCAATG
GCCTCACGTTGGTGTCTAGAGCTCACCAGCTAGTGATGGAGGGATATAAC
TGGTGCCATGACCGGAATGTAGTAACGATTTTCAGTGCTCCAAACTATTG
TTATCGTTGTGGTAACCAAGCTGCAATCATGGAACTTGACGATACTCTAA
AATACTCTTTCTTGCAGTTTGACCCAGCACCTCGTAGAGGCGAGCCACAT
GTTACTCGTCGTACCCCAGACTACTTCCTGTAAGGTACCGCAAGGGCGAA
TTCCAGCACACTGGCGGCCGTTACTAGTGGATCC

Amino Acid Sequence

Protein Sequence of PP2A: MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRC
PVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLL
VALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLF
DYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLW
SDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNW
CHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHV
TRRTPDYFL*

Protein sequence of PTPA : MGSSHHHHHHSQDPAEGERQPPPDSSEEAPPATQNFIIPKKEIHTVPDMG
KWKRSQAYADYIGFILTLNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRW
IDETPPVDQPSRFGNKAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAV
YLKESVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNR
YLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRH
FVDEKAVNENHKDYMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKV
NQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG*

• Vector Type: Bacterial
• Antibiotic: Chloramphenicol
• Full Snapgene Map: pACYCDuet PP2A 6His PTPA203.18 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU3780
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Vector for co-expression of 6His PTPA and PP2A in bacteria.; 2A of PTPA is 15A of expressed protein.; Calculated Molecular weights :; PP2A 35571 Daltons ; 6His PTPA 38232 Daltons
• Low Temp: No