cDNA Clone EWS

• Plasmid: pEGFP-C2-EWS T79A
• Parent Plasmid: pEGFPC2
• Expressed: GFP-EWS T79A
• Genbank: NP_005234
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCA
GGGCTACAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCA
CCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTGAT
GTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTA
TGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTGCTCCAACTGCCC
CCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGAT
ACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTC
TGCATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAG
CCACTGCACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGT
CAACCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGG
ACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGC
CAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAG
CCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCA
ACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATG
GGCAGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAA
GCTCCAAGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGTTC
ATTCCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAGGAGTCTG
GAGGATTTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTGATAAC
CGGGGCAGGGGAAGAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGG
GCGGGGAGGAGGACGCGGTGGAATGGGCAGCGCTGGAGAGCGAGGTGGCT
TCAATAAGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAGGC
CCTCCTGTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACA
AGGATTAAATGACAGTGTGACTCTAGATGATCTGGCAGACTTCTTTAAGC
AGTGTGGGGTTGTTAAGATGAACAAGAGAACTGGGCAACCCATGATCCAC
ATCTACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTGTC
CTATGAAGACCCACCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGA
AAGATTTTCAAGGGAGCAAACTTAAAGTCTCCCTTGCTCGGAAGAAGCCT
CCAATGAACAGTATGCGGGGTGGTCTGCCACCCCGTGAGGGCAGAGGCAT
GCCACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGAC
CCATGGGTCGCATGGGAGGCCGTGGAGGAGATAGAGGAGGCTTCCCTCCA
AGAGGACCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAAACGTCCA
GCACCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCAGA
ACTTCGCCTGGAGAACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAA
GGCTTCCTCCCGCCACCCTTTCCGCCCCCGGGTGGTGATCGTGGCAGAGG
TGGCCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGGTG
GTCCCGGTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTC
CGTGGTGGCCGGGGCATGGACCGAGGTGGCTTTGGTGGAGGAAGACGAGG
TGGCCCTGGGGGGCCCCCTGGACCTTTGATGGAACAGATGGGAGGAAGAA
GAGGAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTCAG
GAGCGCAGAGATCGGCCCTACTAGGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTD
VSYTQAQTTATYGQTAYATSYGQPPTGYTAPTAPQAYSQPVQGYGTGAYD
TTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETS
QPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQ
PTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQ
APSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDN
RGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLG
PPVDPDEDSDNSAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIH
IYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKP
PMNSMRGGLPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPP
RGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPE
GFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGF
RGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQ
ERRDRPY*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU3764
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP for mammalian expression.; Calc Mol Weight : 96594 Daltons