cDNA Clone DAZAP1-b

• Plasmid: pGEX6P-1-6His DAZAP1-b
• Parent Plasmid: pGEX6P1
• Expressed: GST-6His DAZAP1-b
• Genbank: NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCATCATCACCATCACCATATGAACAACTCGGGCGCCGACGAGAT
CGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGCACGACCCAAGAGACTC
TGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTTATCATG
AAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAA
AGACCCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAG
ATGGCCGAAACATCGACCCCAAGCCATGCACACCCCGGGGGATGCAGCCG
GAGAGAACACGGCCGAAGGAAGGATGGCAGAAAGGACCCAGGAGCGATAA
CAGTAAATCAAATAAGATATTTGTCGGTGGAATTCCTCACAATTGTGGTG
AGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACGGAGGTA
GTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTAT
TACTTTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTC
ACGACATCATGGGCAAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGAC
AGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCG
GGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGT
GGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCG
ATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACC
CCCACCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCC
CTCCCCAGGGCTTCCCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTT
GGCTACGGGCCTCCACCTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGT
TCCTCCTCCACCAGCCACTCCCGGGGCAGCACCTCTGGCTTTCCCACCGC
CTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCA
GACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTT
CGGACAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTC
CCTCCGTGCCAGGGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGA
CGAGGGCAGAACCACAACGTGCAAGGGTTCCACCCCTACCGACGCTAGGG
ATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHHHHHHMNNSGADEIGKLF
VGGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNC
VGTVLASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSN
KIFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFED
EQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPN
AANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFT
SYIVSTPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPP
ATPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGF
SDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3762
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/6His tag for bacterial expression.