cDNA Clone RSK3

• Plasmid: pFBHTa RSK3
• Parent Plasmid: pSC-A
• Expressed: 6His-RSK3
• Genbank: CAA59427
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCGACCTGAGCATGAAGAAGTTCGCCGTGCGCAGGTTCTTCTCTGT
GTACCTGCGCAGGAAGTCGCGCTCCAAGAGCTCCAGCCTGAGCCGGCTCG
AGGAAGAAGGTGTCGTGAAGGAGATAGACATCAGCCATCATGTGAAGGAG
GGCTTTGAGAAGGCAGATCCTTCCCAGTTTGAGCTGCTGAAGGTTTTAGG
ACAAGGATCCTATGGAAAGGTGTTCCTGGTGAGGAAGGTGAAGGGGTCCG
ACGCTGGGCAGCTCTACGCCATGAAGGTCCTTAAGAAAGCCACCCTAAAA
GTTCGGGACCGAGTGAGATCGAAGATGGAGAGAGACATCTTGGCAGAAGT
GAATCACCCCTTCATTGTGAAGCTTCATTATGCCTTTCAGACGGAAGGAA
AGCTCTACCTGATCCTGGACTTCCTGCGGGGAGGGGACCTCTTCACCCGG
CTCTCCAAAGAGGTCATGTTCACGGAGGAGGATGTCAAGTTCTACCTGGC
TGAGCTGGCCTTGGCTTTAGACCATCTCCACAGCCTGGGGATCATCTACA
GAGATCTGAAGCCTGAGAACATCCTCCTGGATGAAGAGGGGCACATTAAG
ATCACAGATTTCGGCCTGAGTAAGGAGGCCATTGACCACGACAAGAGAGC
GTACTCCTTCTGCGGGACGATCGAGTACATGGCACCCGAGGTGGTGAACC
GGCGAGGACACACGCAGAGTGCCGACTGGTGGTCCTTCGGCGTGCTCATG
TTTGAGATGCTCACGGGGTCCCTGCCGTTCCAGGGGAAGGACAGGAAGGA
GACCATGGCTCTCATCCTCAAAGCCAAGCTGGGGATGCCGCAGTTCCTCA
GTGGGGAGGCACAGAGTTTGCTGCGAGCTCTCTTCAAACGGAACCCCTGC
AACCGGCTGGGTGCTGGCATTGACGGAGTGGAGGAAATTAAGCGCCATCC
CTTCTTTGTGACCATAGACTGGAACACGCTGTACCGGAAGGAGATCAAGC
CACCGTTCAAACCAGCATTGGGCAGGCCTGAGGACACCTTCCACTTTGAC
CCCGAGTTCACAGCGCGGACGCCCACAGACTCTCCTGGCGTCCCCCCGAG
TGCAAACGCTCATCACCTGTTTAGAGGATTCAGCTTTGTGGCCTCAAGCC
TGATCCAGGAGCCCTCACAGCAAGATCTGCACAAAGTCCCAGTTCACCCA
ATCGTGCAGCAGTTACACGGGAACAACATCCACTTCACCGATGGCTACGA
GATCAAGGAGGACATCGGGGTGGGCTCCTACTCAGTGTGCAAGCGATGTG
TGCATAAAGCCACAGACACCGAGTATGCCGTGAAGATCATTGATAAGAGC
AAGAGAGACCCCTCGGAAGAGATTGAGATCCTCCTGCGGTACGGCCAGCA
CCCGAACATCATCACCCTCAAGGATGTCTATGATGATGGCAAGTTTGTGT
ACCTGGTAATGGAGCTGATGCGTGGTGGGGAGCTCCTGGACCGCATCCTC
CGGCAGAGATACTTCTCGGAGCGCGAAGCCAGTGACGTCCTGTGCACCAT
CACCAAGACCATGGACTACCTCCATTCCCAGGGGGTTGTTCATCGAGACC
TGAAGCCGAGTAACATCCTGTACAGGGATGAGTCGGGGAGCCCAGAATCC
ATCCGAGTCTGCGACTTCGGCTTTGCCAAGCAGCTGCGCGCGGGGAACGG
GCTGCTCATGACACCCTGCTACACGGCCAATTTCGTGGCCCCGGAGGTCC
TGAAGCGTCAAGGCTATGATGCGGCGTGTGACATCTGGAGTTTGGGGATC
CTGTTGTACACCATGCTGGCAGGATTTACCCCTTTTGCAAATGGGCCAGA
CGATACCCCTGAGGAGATTCTGGCGCGGATCGGCAGTGGGAAGTATGCCC
TTTCTGGGGGAAACTGGGACTCGATATCTGACGCAGCTAAAGACGTCGTG
TCCAAGATGCTCCACGTGGACCCTCATCAGCGCCTGACGGCGATGCAAGT
GCTCAAACACCCGTGGGTGGTCAACAGAGAGTACCTGTCCCCAAACCAGC
TCAGCCGACAGGACGTGCACCTGGTGAAGGGCGCGATGGCCGCCACCTAC
TTTGCTCTAAACAGAACACCTCAGGCCCCGCGGCTGGAGCCCGTGCTGTC
ATCCAACCTGGCTCAGCGCAGAGGCATGAAGAGACTCACGTCCACGCGGC
TGTAGGTCGAC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFDLSMKKFAVRRFFSVYLRRK
SRSKSSSLSRLEEEGVVKEIDISHHVKEGFEKADPSQFELLKVLGQGSYG
KVFLVRKVKGSDAGQLYAMKVLKKATLKVRDRVRSKMERDILAEVNHPFI
VKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALA
LDHLHSLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAYSFCG
TIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALI
LKAKLGMPQFLSGEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPFFVTI
DWNTLYRKEIKPPFKPALGRPEDTFHFDPEFTARTPTDSPGVPPSANAHH
LFRGFSFVASSLIQEPSQQDLHKVPVHPIVQQLHGNNIHFTDGYEIKEDI
GVGSYSVCKRCVHKATDTEYAVKIIDKSKRDPSEEIEILLRYGQHPNIIT
LKDVYDDGKFVYLVMELMRGGELLDRILRQRYFSEREASDVLCTITKTMD
YLHSQGVVHRDLKPSNILYRDESGSPESIRVCDFGFAKQLRAGNGLLMTP
CYTANFVAPEVLKRQGYDAACDIWSLGILLYTMLAGFTPFANGPDDTPEE
ILARIGSGKYALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPW
VVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQ
RRGMKRLTSTRL

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3753
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for baculovirus expression of N terminal 6His tag.; Calc Mol Weight : 86834 Daltons