cDNA Clone DAZAP1-b

• Plasmid: pGEX6P-1-6His DAZAP1-b 155-407 T269A T315A
• Parent Plasmid: pGEX6P1
• Expressed: GST-6His DAZAP1-b 155-407 T269A T315A
• Genbank: NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCATCATCACCATCACCATGGTTTTGGATTTATTACTTTCGAGGA
CGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGG
GCAAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAA
GCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAA
CGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGAT
ATGGCCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTAT
GGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCAC
CTCCTACATCGTGTCCGCCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCT
TCCCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCT
CCACCTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACC
AGCCGCTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGG
CTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTAT
GGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTT
CTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAG
GGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAAC
CACAACGTGCAAGGGTTCCACCCCTACCGACGCTAGGGATCCGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHHHHHHGFGFITFEDEQSV
DQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANG
WAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIV
SAPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPAAPG
AAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPS
QQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3752
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/6His tag for bacterial expression.; Calc Mol Weight : 53588 Daltons