cDNA Clone SGK1

• Plasmid: pEBG6P-SGK1
• Parent Plasmid: pEBG6P
• Expressed: GST-SGK1
• Genbank: XP_037046
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACGGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTC
CAGGATGAGGGGCATGGTGGCAATTCTCATCGCTTTCATGAAGCAGAGGA
GGATGGGTCTGAACGACTTTATTCAGAAGATTGCCAATAACTCCTATGCA
TGCAAACACCCTGAAGTTCAGTCCATCTTGAAGATCTCCCAACCTCAGGA
GCCTGAGCTTATGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCTCAGC
AAATCAACCTTGGCCCGTCGTCCAATCCTCATGCTAAACCATCTGACTTT
CACTTCTTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGC
AAGACACAAGGCAGAAGAAGTGTTCTATGCAGTCAAAGTTTTACAGAAGA
AAGCAATCCTGAAAAAGAAAGAGGAGAAGCATATTATGTCGGAGCGGAAT
GTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTC
TTTCCAGACTGCTGACAAATTGTACTTTGTCCTAGACTACATTAATGGTG
GAGAGTTGTTCTACCATCTCCAGAGGGAACGCTGCTTCCTGGAACCACGG
GCTCGTTTCTATGCTGCTGAAATAGCCAGTGCCTTGGGCTACCTGCATTC
ACTGAACATCGTTTATAGAGACTTAAAACCAGAGAATATTTTGCTAGATT
CACAGGGACACATTGTCCTTACTGATTTCGGACTCTGCAAGGAGAACATT
GAACACAACAGCACAACATCCACCTTCTGTGGCACGCCGGAGTATCTCGC
ACCTGAGGTGCTTCATAAGCAGCCTTATGACAGGACTGTGGACTGGTGGT
GCCTGGGAGCTGTCTTGTATGAGATGCTGTATGGCCTGCCGCCTTTTTAT
AGCCGAAACACAGCTGAAATGTACGACAACATTCTGAACAAGCCTCTCCA
GCTGAAACCAAATATTACAAATTCCGCAAGACACCTCCTGGAGGGCCTCC
TGCAGAAGGACAGGACAAAGCGGCTCGGGGCCAAGGATGACTTCATGGAG
ATTAAGAGTCATGTCTTCTTCTCCTTAATTAACTGGGATGATCTCATTAA
TAAGAAGATTACTCCCCCTTTTAACCCAAATGTGAGTGGGCCCAACGACC
TACGGCACTTTGACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATT
GGCAAGTCCCCTGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGC
CGAGGCTTTCCTAGGCTTTTCCTATGCGCCTCCCACGGACTCTTTCCTCT
GAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMTVKTEAAKGTLT
YSRMRGMVAILIAFMKQRRMGLNDFIQKIANNSYACKHPEVQSILKISQP
QEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVL
LARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLH
FSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYL
HSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEY
LAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKP
LQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDL
INKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSIGKSPDSVLVTASVKE
AAEAFLGFSYAPPTDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3740
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.