cDNA Clone OSM

• Plasmid: pEBG6P-OSM
• Parent Plasmid: pEBG6P
• Expressed: GST-OSM
• Genbank: NP_113631
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGAGGAGGGCAAGAAGGGCAAGAAGCCTGGAATTGTCTC
GCCATTTAAACGAGTATTCCTAAAAGGTGAAAAGAGTAGAGATAAGAAAG
CCCATGAGAAGGTGACAGAGAGGCGCCCTCTGCACACTGTGGTGTTGTCA
TTGCCTGAGCGCGTCGAGCCAGACAGACTGCTGAGCGACTATATTGAGAA
GGAGGTAAAGTATTTAGGTCAGTTAACGTCCATACCAGGATACCTGAATC
CCTCCAGTAGGACTGAAATCCTGCATTTCATAGACAATGCAAAGAGAGCC
CACCAGCTTCCGGGACACTTGACTCAGGAGCACGATGCTGTGCTCAGCCT
GTCTGCGTACAACGTCAAGCTGGCCTGGAGGGACGGGGAGGATATCATCC
TCAGGGTGCCCATCCATGACATCGCCGCCGTCTCCTATGTTCGGGATGAC
GCTGCACACCTGGTGGTCCTGAAGACAGCCCAGGACCCAGGGATCTCCCC
CAGCCAGAGTCTGTGTGCGGAAAGTTCCAGAGGCCTCAGTGCAGGCTCCC
TGTCGGAGAGTGCAGTTGGGCCCGTGGAGGCATGCTGCCTGGTCATCCTG
GCTGCAGAGAGCAAGGTCGCTGCGGAGGAGCTTTGCTGTCTGCTAGGCCA
GGTCTTCCAGGTTGTTTACACGGAGTCCACCATCGACTTTCTGGACAGAG
CGATATTTGATGGGGCCTCTACCCCGACCCACCACCTGTCCCTGCACAGC
GATGACTCTTCTACAAAAGTGGACATTAAGGAGACCTACGAGGTGGAAGC
CAGCACTTTCTGCTTCCCTGAATCTGTGGATGTGGGTGGTGCATCACCCC
ACAGCAAGACCATCAGTGAGAGCGAGCTGAGCGCCAGCGCCACTGAGCTG
CTGCAGGACTACATGCTGACGCTGCGCACCAAGCTGTCATCACAGGAGAT
CCAGCAGTTTGCAGCACTGCTGCACGAGTACCGCAATGGGGCCTCTATCC
ACGAGTTCTGCATCAACCTGCGGCAGCTCTACGGGGACAGCCGCAAGTTC
CTGCTGCTTGGTCTGAGGCCCTTCATCCCTGAGAAGGACAGCCAGCACTT
CGAGAACTTCCTGGAGACCATTGGCGTGAAGGATGGCCGCGGCATCATCA
CTGACAGCTTTGGCAGGCACCGGCGGGCCCTGAGCACCACATCCAGTTCC
ACCACCAATGGGAACAGGGCCACGGGCAGCTCTGATGACCGGTCGGCACC
CTCAGAGGGGGATGAGTGGGACCGCATGATCTCGGACATCAGCAGCGACA
TTGAGGCGCTGGGCTGCAGCATGGACCAGGACTCAGCATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEEEGKKGKKPGI
VSPFKRVFLKGEKSRDKKAHEKVTERRPLHTVVLSLPERVEPDRLLSDYI
EKEVKYLGQLTSIPGYLNPSSRTEILHFIDNAKRAHQLPGHLTQEHDAVL
SLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDPGI
SPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAAESKVAAEELCCLL
GQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKETYEV
EASTFCFPESVDVGGASPHSKTISESELSASATELLQDYMLTLRTKLSSQ
EIQQFAALLHEYRNGASIHEFCINLRQLYGDSRKFLLLGLRPFIPEKDSQ
HFENFLETIGVKDGRGIITDSFGRHRRALSTTSSSTTNGNRATGSSDDRS
APSEGDEWDRMISDISSDIEALGCSMDQDSA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3695
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.