cDNA Clone PHK muscle

• Plasmid: pFBHTb PBK muscle 1-297
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PBK muscle 1-297
• Genbank: X80590
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCACCCGGGACGAGGCACTGCCGGACTCTCATTCTGCACAGGACTT
CTATGAGAATTATGAGCCCAAAGAGATCCTGGGCAGGGGCGTTAGCAGTG
TGGTCAGGCGATGCATCCACAAGCCCACGAGCCAGGAGTACGCCGTGAAG
GTCATCGACGTCACCGGTGGAGGCAGCTTCAGCCCGGAGGAGGTGCGGGA
GCTGCGAGAAGCCACGCTGAAGGAGGTGGACATCCTGCGCAAGGTCTCAG
GGCACCCCAACATCATACAGCTGAAGGACACTTATGAGACCAACACTTTC
TTCTTCTTGGTGTTTGACCTGATGAAGAGAGGGGAGCTCTTTGACTACCT
CACTGAGAAGGTCACCTTGAGTGAGAAGGAAACCAGAAAGATCATGCGAG
CTCTGCTGGAGGTGATCTGCACCTTGCACAAACTCAACATCGTGCACCGG
GACCTGAAGCCCGAGAACATTCTCTTGGATGACAACATGAACATCAAGCT
CACAGACTTTGGCTTTTCCTGCCAGCTGGAGCCGGGAGAGAGGCTGCGAG
AGGTCTGCGGGACCCCCAGTTACCTGGCCCCTGAGATTATCGAGTGCTCC
ATGAATGAGGACCACCCGGGCTACGGGAAAGAGGTGGACATGTGGAGCAC
TGGCGTCATCATGTACACGCTGCTGGCCGGCTCCCCGCCCTTCTGGCACC
GGAAGCAGATGCTGATGCTGAGGATGATCATGAGCGGCAACTACCAGTTT
GGCTCGCCCGAGTGGGATGATTACTCGGACACCGTGAAGGACCTGGTCTC
CCGATTCCTGGTGGTGCAACCCCAGAACCGCTACACAGCGGAAGAGGCCT
TGGCACACCCCTTCTTCCAGCAGTACTTGGTGGAGGAAGTGCGGTAAGAA
TTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSTRDEALPDSHSAQDFYENYEPK
EILGRGVSSVVRRCIHKPTSQEYAVKVIDVTGGGSFSPEEVRELREATLK
EVDILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLS
EKETRKIMRALLEVICTLHKLNIVHRDLKPENILLDDNMNIKLTDFGFSC
QLEPGERLREVCGTPSYLAPEIIECSMNEDHPGYGKEVDMWSTGVIMYTL
LAGSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDLVSRFLVVQP
QNRYTAEEALAHPFFQQYLVEEVR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3679
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6HIS tag.