cDNA Clone Ubiquitin

• Plasmid: pCMV5 STREP P 6His HA Ubiquitin K48R
• Parent Plasmid: pCMV5 strep 3C 6His
• Expressed: STREP P 6His HA Ubiquitin K48R
• Genbank: P62988
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGATCTT
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MASSLRQILDSQKMEWRSNAGGSELALEVLFQGPWHHHHHHGSATMYPYD
VPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
GRQLEDGRTLSDYNIQKESTLHLVLRLRGG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3677
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal STREP/6His/HA tag for mammalian expression. (Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7480-5)