cDNA Clone DAZAP1-b

• Plasmid: pET28a DAZAP1-b 155-407 T269A T315A
• Parent Plasmid: pET28a original
• Expressed: 6His-DAZAP1-b 155-407 T269A T315A
• Genbank: NM_018959
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTGGACCA
GGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTA
AACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGT
GCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGC
AGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGT
GGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGA
AGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTGTCCGC
CCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTACGGTG
CCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGCCAGAT
CAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGCTCCCGGGGCAGC
ACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCA
AGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTAC
GGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGCCAGCA
GCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCCCCCCG
CCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAAGGGTTC
CACCCCTACCGACGCTAGGGATCC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSGFGFITFEDEQSVDQA
VNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANGWAG
QPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSAPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPAAPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU3667
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for bacterial expression.