cDNA Clone PRAK

• Synonyms: MAPKAPK5
• Plasmid: pGEX-PRAK
• Parent Plasmid: pGEX4T1
• Expressed: GST-PRAK
• Genbank: BC041049
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCCGGGAATTTCCGGTGGTGGTGGTGGAATTCATATGTCGGAGGA
GAGCGACATGGACAAAGCCATCAAGGAAACTTCCATTTTAGAAGAATACA
GTATCAATTGGACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGA
GTCTGTGTAAAGAAATCTACTCAAGAACGGTTTGCGCTGAAAATTCTTCT
TGATCGTCCAAAAGCTAGAAATGAGGTACGTCTGCACATGATGTGTGCCA
CACACCCAAACATAGTTCAGATTATTGAAGTGTTTGCTAACAGTGTCCAG
TTTCCCCATGAGTCCAGCCCTAGGGCCCGACTCTTAATTGTAATGGAGAT
GATGGAAGGGGGAGAGCTATTTCACAGAATCAGCCAGCACCGGCACTTTA
CAGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCTTTGGCTCTGCGG
CACTGTCACTTGTTAAACATTGCGCACAGAGACCTCAAGCCTGAAAATCT
GCTTTTTAAGGATAACTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTG
GATTTGCCAAGATTGACCAAGGTGACTTGATGACACCCCAGTTCACCCCT
TATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGA
GAAATCTGGCATCATACCTACCTCACCGACGCCCTACACTTACAACAAGA
GCTGTGACTTGTGGTCCCTAGGGGTGATTATCTATGTGATGCTGTGCGGA
TACCCTCCTTTTTACTCCAAACACCACAGCCGGACTATCCCAAAGGATAT
GCGAAGAAAGATCATGACAGGCAGTTTTGAGTTCCCAGAGGAAGAGTGGA
GTCAGATCTCAGAGATGGCCAAAGATGTTGTGAGGAAGCTCCTGAAGGTC
AAACCGGAGGAGAGACTCACCATCGAGGGAGTGCTGGACCACCCCTGGCT
CAATTCCACCGAGGCCCTGGATAATGTGCTGCCTTCTGCTCAGCTGATGA
TGGACAAGGCAGTGGTTGCAGGAATCCAGCAGGCTCACGCGGAACAGTTG
GCCAACATGAGAATCCAGGATCTGAAAGTCAGCCTCAAACCCCTGCACTC
AGTGAACAACCCCATTCTGCGGAAGAGGAAGTTACTTGGCACCAAGCCAA
AGGACAGTGTCTATATCCACGACCATGAGAATGGAGCCGAGGATTCCAAT
GTTGCCTTGGAAAAACTCCGAGATGTGATTGCTCAGTGTATTCTCCCCCA
GGCTGGAGAGAATGAAGATGAGAAACTGAATGAAGTAATGCAGGAGGCTT
GGAAGTATAACCGGGAATGCAAACTCCTAAGAGATACTCTGCAGAGCTTC
AGCTGGAATGGTCGTGGATTCACAGATAAAGTAGATCGACTAAAACTGGC
AGAAATTGTGAAGCAGGTGATAGAAGAGCAAACCACGTCCCACGAATCCC
AATAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPGISGGGGGIHMSEESDMDKAIKE
TSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEV
RLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHR
ISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNSLDA
PVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSP
TPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRRKIMTGSF
EFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNV
LPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKR
KLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGENEDEKL
NEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEE
QTTSHESQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3636
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.