cDNA Clone OGFR

• Plasmid: pCMV5-HA OGFR S299A
• Parent Plasmid: pCMV5
• Expressed: HA-OGFR S299A
• Genbank: NM_007346
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGACGA
CCCCGACTGCGACTCCACCTGGGAGGAGGACGAGGAGGATGCGGAGGACG
CGGAGGACGAGGACTGCGAGGACGGCGAGGCCGCCGGCGCGAGGGACGCG
GACGCAGGGGACGAGGACGAGGAGTCGGAGGAGCCGCGGGCGGCGCGGCC
CAGCTCGTTCCAGTCCAGAATGACAGGGTCCAGAAACTGGCGAGCCACGA
GGGACATGTGTAGGTATCGGCACAACTATCCGGATCTGGTGGAACGAGAC
TGCAATGGGGACACGCCAAACCTGAGTTTCTACAGAAATGAGATCCGCTT
CCTGCCCAACGGCTGTTTCATTGAGGACATTCTTCAGAACTGGACGGACA
ACTATGACCTCCTTGAGGACAATCACTCCTACATCCAGTGGCTGTTTCCT
CTGCGAGAACCAGGAGTGAACTGGCATGCCAAGCCCCTCACGCTCAGGGA
GGTCGAGGTGTTTAAAAGCTCCCAGGAGATCCAGGAGCGGCTTGTCCGGG
CCTACGAGCTCATGCTGGGCTTCTACGGGATCCGGCTGGAGGACCGAGGC
ACGGGCACGGTGGGCCGAGCACAGAACTACCAGAAGCGCTTCCAGAACCT
GAACTGGCGCAGCCACAACAACCTCCGCATCACACGCATCCTCAAGTCGC
TGGGTGAGCTGGGCCTCGAGCACTTCCAGGCGCCGCTGGTCCGCTTCTTC
CTGGAGGAGACGCTGGTGCGGCGGGAGCTGCCGGGGGTGCGGCAGAGTGC
CCTGGACTACTTCATGTTCGCCGTGCGCTGCCGACACCAGCGCCGCCAGC
TGGTGCACTTCGCCTGGGAGCACTTCCGGCCCCGCTGCAAGTTCGTCTGG
GGGCCCCAAGACAAGCTGCGGAGGTTCAAGCCCAGCGCTCTGCCCCATCC
GCTCGAGGGCTCCAGGAAGGTGGAGGAGGAAGGAAGCCCCGGGGACCCCG
ACCACGAGGCCAGCACCCAGGGTCGGACCTGTGGGCCAGAGCATAGCAAG
GGTGGGGGCAGGGTGGACGAGGGGCCCCAGCCACGGAGCGTGGAGCCCCA
GGATGCGGGACCCCTGGAGAGGAGCCAGGGGGATGAGGCAGGGGGCCACG
GGGAAGATAGGCCGGAGCCCTTAAGCCCCAAAGAGAGCAAGAAGAGGAAG
CTGGAGCTGAGCCGGCGGGAGCAGCCGCCCACAGAGCCAGGCCCTCAGAG
TGCCTCAGAGGTGGAGAAGATCGCTCTGAATTTGGAGGGGTGTGCCCTCA
GCCAGGGCAGCCTCAGGACGGGGACCCAGGAAGTGGGCGGTCAGGACCCT
GGGGAGGCAGTGCAGCCCTGCCGCCAACCCCTGGGAGCCAGGGTGGCCGA
CAAGGTGAGGAAGCGGAGGAAGGTGGATGAGGGTGCTGGGGACAGTGCTG
CGGTGGCCAGTGGTGGTGCCCAGACCTTGGCCCTTGCCGGGTCCCCTGCC
CCATCGGGGCACCCCAAGGCTGGACACAGTGAGAACGGGGTTGAGGAGGA
CACAGAAGGTCGAACGGGGCCCAAAGAAGGTACCCCTGGGAGCCCATCGG
AGACCCCAGGCCCCAGCCCAGCAGGACCTGCAGGGGACGAGCCAGCCGAG
AGCCCATCGGAGACCCCAGGCCCCCGCCCAGCAGGACCTGCAGGGGACGA
GCCGGCCGAGAGCCCATCGGAGACCCCAGGCCCCCGCCCAGCAGGACCTG
CAGGGGACGAGCCAGCCAAGACCCCATCGGAGACCCCAGGCCCCAGCCCG
GCAGGACCTACAAGGGATGAGCCAGCCGAGAGCCCATCGGAGACCCCAGG
CCCCCGCCCGGCAGGACCTGCAGGGGACGAGCCAGCCGAGAGCCCATCGG
AGACCCCAGGCCCCCGCCCGGCAGGACCTGCAGGGGACGAGCCAGCCGAG
AGCCCATCGGAGACCCCAGGCCCCAGCCCGGCAGGACCTACAAGGGATGA
GCCAGCCAAGGCGGGGGAGGCAGCAGAGTTGCAGGACGCAGAGGTGGAGT
CTTCTGCCAAGTCTGGGAAGCCTTAAAGATCT

Amino Acid Sequence

MYPYDVPDYAMDDPDCDSTWEEDEEDAEDAEDEDCEDGEAAGARDADAGD
EDEESEEPRAARPSSFQSRMTGSRNWRATRDMCRYRHNYPDLVERDCNGD
TPNLSFYRNEIRFLPNGCFIEDILQNWTDNYDLLEDNHSYIQWLFPLREP
GVNWHAKPLTLREVEVFKSSQEIQERLVRAYELMLGFYGIRLEDRGTGTV
GRAQNYQKRFQNLNWRSHNNLRITRILKSLGELGLEHFQAPLVRFFLEET
LVRRELPGVRQSALDYFMFAVRCRHQRRQLVHFAWEHFRPRCKFVWGPQD
KLRRFKPSALPHPLEGSRKVEEEGSPGDPDHEASTQGRTCGPEHSKGGGR
VDEGPQPRSVEPQDAGPLERSQGDEAGGHGEDRPEPLSPKESKKRKLELS
RREQPPTEPGPQSASEVEKIALNLEGCALSQGSLRTGTQEVGGQDPGEAV
QPCRQPLGARVADKVRKRRKVDEGAGDSAAVASGGAQTLALAGSPAPSGH
PKAGHSENGVEEDTEGRTGPKEGTPGSPSETPGPSPAGPAGDEPAESPSE
TPGPRPAGPAGDEPAESPSETPGPRPAGPAGDEPAKTPSETPGPSPAGPT
RDEPAESPSETPGPRPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSE
TPGPSPAGPTRDEPAKAGEAAELQDAEVESSAKSGKP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3566
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression. Amplified from BC014137 (published seq is incorrect and gives above which has E576K S577T (c.f. BC032666)