cDNA Clone TAK1-S

• Plasmid: pFBHTb TAK1 1-303 TAB1 371-504 S423A T431A
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TAK1 1-303 TAB1 371-504 S423A T431A
• Genbank: NM_003188 (TAK1) MAP3K7IP1 (TAB1)
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGC
CGGTGAGATGATCGAAGCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCG
ACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGAAGAGGAGCCTTTGGA
GTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCTATTAAACAAAT
AGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAGTTAT
CCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAAT
CCAGTGTGTCTTGTGATGGAATATGCTGAAGGGGGCTCTTTATATAATGT
GCTGCATGGTGCTGAACCATTGCCATATTATACTGCTGCCCACGCAATGA
GTTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTATCTTCACAGCATGCAA
CCCAAAGCGCTAATTCACAGGGACCTGAAACCACCAAACTTACTGCTGGT
TGCAGGGGGGACAGTTCTAAAAATTTGTGATTTTGGTACAGCCTGTGACA
TTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCT
GAAGTTTTTGAAGGTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTG
GGGTATTATTCTTTGGGAAGTGATAACGCGTCGGAAACCCTTTGATGAGA
TTGGTGGCCCAGCTTTCCGAATCATGTGGGCTGTTCATAATGGTACTCGA
CCACCACTGATAAAAAATTTACCTAAGCCCATTGAGAGCCTGATGACTCG
TTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAGGAAATTGTGA
AAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTA
CAGTATCCTTGTCAGGAATTCGGAGGAGGAGGAGGAGAAATGAGCCAGCC
CACACCGAGCCCAGCCCCAGCTGCAGGAGGACGAGTGTACCCTGTGTCTG
TGCCATACTCCAGCGCCCAGAGCACCAGCAAGACCAGCGTGACCCTCTCC
CTTGTCATGCCCTCCCAGGGCCAGATGGTCAACGGGGCTCACGCTGCTTC
CACCCTGGACGAAGCCGCCCCCACCCTCACCAACCAAAGCCCGACCTTAA
CCCTGCAGTCCACCAACACGCACACGCAGAGCAGCAGCTCCAGCTCTGAC
GGAGGCCTCTTCCGCTCCCGGCCCGCCCACTCGCTCCCGCCTGGCGAGGA
CGGTCGTGTTGAGCCCTATGTGGACTTTGCTGAGTTTTACCGCCTCTGGA
GCGTGGACCATGGCGAGCAGAGCGTGGTGACAGCACCGTAGGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMSTASAASSSSSSSAGEMIEAP
SQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERK
AFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPL
PYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLK
ICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEV
ITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPS
QRPSMEEIVKIMTHLMRYFPGADEPLQYPCQEFGGGGGEMSQPTPSPAPA
AGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQGQMVNGAHAASTLDEAAP
TLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPGEDGRVEPYV
DFAEFYRLWSVDHGEQSVVTAP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3537
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression.