cDNA Clone NEDD4-2

• Plasmid: pEBG6P-Nedd4-2
• Parent Plasmid: pEBG6P
• Expressed: GST-Nedd4-2
• Genbank: AY112985
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAGCGACC
CTATACATTTAAGGACTTTCTCCTCAGACCAAGAAGTCATAAGTCTCGAG
TTAAGGGATTTTTGCGATTGAAAATGGCCCATATGCCAAAAAATGGAGGT
CAAGATGAAGAAAACAGTGACCAGAGGGATGACATGGAGCATGGATGGGA
AGTTGTTGACTCAAATGACTCGGCTTCTCAGCACCAAGAGGAACTTCCTC
CTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAGTGAACAATTTAGGCCGA
ACTTACTATGTCAACCACAACAACCGGACCACTCAGTGGCACAGACCAAG
CCTGATGGACGTGTCCTCGGAGTCGGACAATAACATCAGACAGATCAACC
AGGAGGCAGCACACCGGCGCTTCCGCTCCCGCAGGCACATCAGCGAAGAC
TTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTCCCCGAGCCTTGGGAGAC
CATTTCAGAGGAAGTGAATATCGCTGGAGACTCTCTCGGTCTGGCTCTGC
CCCCACCACCGGCCTCCCCAGGATCTCGGACCAGCCCTCAGGAGCTGTCA
GAGGAACTATGCAGAAGGCTTCAGATCACTCCAGACTCCAATGGGGAACA
GTTCAGCTCTTTGATTCAAAGAGAACCCTCCTCAAGGTTGAGGTCATGCA
GTGTCACCGACGCAGTTGCAGAACAGGGCCATCTACCACCGCCATCAGTG
GCCTATGTACATACCACGCCGGGTCTGCCCTCAGGCTGGGAAGAAAGAAA
AGATGCTAAGGGGCGCACATACTATGTCAATCATAACAATCGAACCACAA
CTTGGACTCGACCTATCATGCAGCTTGCAGAAGATGGTGCGTCCGGATCA
GCCACAAACAGTAACAACCATCTAATCGAGCCTCAGATCCGCCGGCCTCG
TAGCCTCAGCTCGCCAACAGTAACTTTATCTGCCCCGCTGGAGGGTGCCA
AGGACTCACCCGTACGTCGGGCTGTGAAAGACACCCTTTCCAACCCACAG
TCCCCACAGCCATCACCTTACAACTCCCCCAAACCACAACACAAAGTCAC
ACAGAGCTTCTTGCCACCCGGCTGGGAAATGAGGATAGCGCCAAACGGCC
GGCCCTTCTTCATTGATCATAACACAAAGACTACAACCTGGGAAGATCCA
CGTTTGAAATTTCCAGTACATATGCGGTCAAAGACATCTTTAAACCCCAA
TGACCTTGGCCCCCTTCCTCCTGGCTGGGAAGAAAGAATTCACTTGGATG
GCCGAACGTTTTATATTGATCATAATAGCAAAATTACTCAGTGGGAAGAC
CCAAGACTGCAGAACCCAGCTATTACTGGTCCGGCTGTCCCTTACTCCAG
AGAATTTAAGCAGAAATATGACTACTTCAGGAAGAAATTAAAGAAACCTG
CTGATATCCCCAATAGGTTTGAAATGAAACTTCACAGAGATAACATATTT
GAAGAGTCCTATCGGAGAATTATGTCCGTGAAAAGACCAGATGTCCTAAA
AGCTAGACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTATGGGG
GTGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTCCAACCCC
TACTACGGCCTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGAT
CAACCCTAATTCAGGCCTCTGTAATGAGGATCATTTGTCCTACTTCACTT
TTATTGGAAGAGTTGCTGGTCTGGCCGTATTTCATGGGAAGCTCTTAGAT
GGTTTCTTCATTAGACCATTTTACAAGATGATGTTGGGAAAGCAGATAAC
CCTGAATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTGAAAT
GGATCCTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGAC
GAAGAAAACTTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTC
AGAAATAATGGTCACAAATGAAAACAAAAGGGAATATATCGACTTAGTCA
TCCAGTGGAGATTTGTGAACAGGGTCCAGAAGCAGATGAACGCCTTCTTG
GAGGGATTCACAGAACTACTTCCTATTGATTTGATTAAAATTTTTGACGA
AAATGAGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTGAATG
ACTGGAGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCC
GTCATTCAGTGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCG
TATCCGGTTACTGCAGTTTGTCACAGGGACATCGCGAGTACCTATGAATG
GATTTGCCGAACTTTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAG
CAATGGGGCAGTCCTGAGAAACTGCCCAGAGCTCACACATGCTTTAATCG
CCTTGACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAACTTC
TCATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAAAGATCT

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSATMYPYDVPDYAE
RPYTFKDFLLRPRSHKSRVKGFLRLKMAHMPKNGGQDEENSDQRDDMEHG
WEVVDSNDSASQHQEELPPPPLPPGWEEKVNNLGRTYYVNHNNRTTQWHR
PSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPW
ETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELCRRLQITPDSNG
EQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSVAYVHTTPGLPSGWEE
RKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRR
PRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHK
VTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLN
PNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPY
SREFKQKYDYFRKKLKKPADIPNRFEMKLHRDNIFEESYRRIMSVKRPDV
LKARLWIEFESEKGLDYGGVAREWFFLLSKEMSNPYYGLFEYSATDNYTL
QINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQ
ITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPN
GSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIF
DENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAE
KRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCF
NRLDLPPYETFEDLREKLLMAVENAQGFEGVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3528
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST/HA tag.