cDNA Clone TAK1-S

• Plasmid: pFBHTb TAK1 1-303 TAB1 371-504 T431A
• Parent Plasmid: pEBG6P
• Expressed: 6His-TAK1 1-303 TAB1 371-504 T431A
• Genbank: AY112985
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAG
AAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCCATA
TGCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGAC
ATGGAGCATGGATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCA
CCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAG
TGAACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACT
CAGTGGCACAGACCGAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAA
CATCAGACAGATCAACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCA
GGCACATCAGCGAAGACTTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTC
CCCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAATATCGCTGGAGACCC
TCTCGGTCTGGCTCTGCCCCCACCACCGGCCTCCCCAGGATCTCGGACCA
GCCCTCAGGAGCTGTCAGAGGAACTATGCAGAAGGCTTCAGATCACTCCA
GACTCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTC
AAGGTTGAGGTCATGCAGTGTCACCGACGCAGTTGCAGAACAGGGCCATC
TACCACCGCCATCAGTGGCCTATGTACATACCACGCCGGGTCTGCCCTCA
GGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACATACTATGTCAATCA
TAACAATCGAACCACAACTTGGACTCGACCTATCATGCAGCTTGCAGAAG
ATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCTAATCGAGCCT
CAGATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAACTTTATCTGC
CCCGCTGGAGGGTGCCAAGGACTCACCCGTACGTCGGGCTGTGAAAGACA
CCCTTTCCAACCCACAGTCCCCACAGCCATCACCTTACAACTCCCCCAAA
CCACAACACAAAGTCACACAGAGCTTCTTGCCGCCCGGCTGGGAAATGAG
GATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAACACAAAGACTA
CAACCTGGGAAGATCCACGTTTGAAGTTTCCAGTACATATGCGGTCAAAG
ACATCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTGGCTGGGAAGA
AAGAATTCACTTGGATGGCCGAACGTTTTATATTGATCATAATAGCAAAA
TTACTCAGTGGGAAGACCCAAGACTGCAGAACCCAGCTATTACTGGTCCG
GCTGTCCCTTACTCCAGAGAATTTAAGCAGAAATATGACTACTTCAGGAA
GAAATTAAAGAAACCTGCTGATATCCCCAATAGGTTTGAAATGAAACTTC
ACAGAGATAACATATTTGAAGAGTCCTATCGGAGAATTATGTCCGTGAAA
AGACCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTGAATCAGAGAA
AGGTCTTGACTATGGGGGTGTGGCCAGAGAATGGTTCTTCTTACTGTCCA
AAGAGATGTTCAACCCCTACTACGGCCTCTTTGAGTACTCTGCCACGGAC
AACTACACCCTTCAGATCAACCCTAATTCAGGCCTCTGTAATGAGGATCA
TTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTGGCCGTATTTC
ATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTACAAGATGATG
TTGGGAAAGCAGATAACCCTGAACGACATGGAATCTGTGGATAGTGAATA
TTACAACTCTTTGAAATGGATCCTGGAGAATGACCCTACTGAGCTGGACC
TCATGTTCTGCATAGACGAAGAAAACTTTGGACAGACATATCAAGTGGAT
TTGAAGCCCAATGGGTCAGAAATAATGGTCACAAATGAAAACAAAAGGGA
ATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGGGTCCAGAAGC
AGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCCTATTGATTTG
ATTAAAATTTTTGACGAAAATGAGCTGGAGTTGCTCATGTGCGGCCTCGG
TGATGTGGATGTGAATGACTGGAGACAGCATTCTATTTACAAGAACGGCT
ACTGCCCAAACCACCCCGTCATTCAGTGGTTCTGGAAGGCTGTGCTACTC
ATGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTTGTCACAGGGACATC
GCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCCAATGGTCCTC
AGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACTGCCCAGAGCT
CACACATGCTTTAATCGCCTTGACTTACCTCCATATGAAACCTTTGAAGA
TTTACGAGAGAAACTTCTCATGGCCGTGGAAAATGCTCAAGGATTTGAAG
GGGTGGATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSATMYPYDVPDYAE
RPYTFKDFLLRPRSHKSRVKGFLRLKMAHMPKNGGQDEENSDQRDDMEHG
WEVVDSNDSASQHQEELPPPPLPPGWEEKVNNLGRTYYVNHNNRTTQWHR
PSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPW
ETISEEVNIAGDPLGLALPPPPASPGSRTSPQELSEELCRRLQITPDSNG
EQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSVAYVHTTPGLPSGWEE
RKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRR
PRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHK
VTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLN
PNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPY
SREFKQKYDYFRKKLKKPADIPNRFEMKLHRDNIFEESYRRIMSVKRPDV
LKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTL
QINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQ
ITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPN
GSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIF
DENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAE
KRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCF
NRLDLPPYETFEDLREKLLMAVENAQGFEGVD*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3505
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag.